| BackgroundWound healing is the general name for the pathologic process caused by wound, and it is composed of inflammatory reaction, granulation tissue hyperplasia, wound re-epithdialization, and the remodeling after wound concrescence.The issue of wound healing has been one of the focuses in clinical surgery. Some factors can result in the failure of wound healing for a long period, so it is an intractable trouble in clinic treatment. In the recent years, many new concepts and therapies concerning wound healing have occurred home and abroad, and to a large extent they proposed new challenges to the traditional wound healing therapies. The field of the Chinese traditional medicine for wound healing take this opportunity and become hot as well. At present, among those most researched Chinese traditional medicine for wound healing ,there are Shengji Yuhong Gao, an ancient recipe, and Qufu Shengji Gao, a testified recipe. The functioning of these recipes are mainly through the following aspects: the growing and activation of fibroblasts, the chemotaxis of macrophages, the oxidation metabolism enhancing process of the cells in the wound, the raising of the fibronection content during wound healing, and accelerating of the growing of the contractive substance in the wound, etc.Ecdysterone (EDS) is a kind of steroid compound extensively existing in the nature. EDS is a hormone regulating the growing and metabolism of insects and was first studied by entomologists. Afterwards, a lot of similar substances were separated from many kinds of plants. It is found that the distribution of EDS in plants is more and broader than in animals, with a rich resource in plants. EDS also expresses strong pharmacological vigor on higher animals, such as the accelerating of protein synthesizing, the regulation of glucolipide metabolism, the regulation of immunity, influencing the central cholinergic neurotransmitter metabolism, etc. However, it is rarely reported whether EDS has an ability to accelerate the wound healing process. Therefore, as a remedy monomer with an extensive source, EDS, if proved to possess an ability to accelerate wound healing, will have a higher promotion and application value than complex drugs for wound healing.ObjectiveTo study the efficacy of ecdysterone in promoting wound healing in rabbits.MothodsTwenty-one New Zealand rabbits were randomized equally into 3 groups and in each rabbits, 4 separate full-thickness skin wounds were induced and treated with dexamethasone, ecdysterone and Yunnan white power, or untreated, respectively. On day 3, 6, and 9, respectively, one group of rabbits were sacrificed and calculated the healing rates. Obtained part of the tissues in the wound; separately made routine sections and super-thin sections, and compared the differences between the effects of different treatment factors in different times with optics microscope and electric microscope. Utilized software SPSS13.0 to compare the mean of various samples by block factorial design and analysis of variance.ResultsThree days after the operation,the 4 wounds in each rabbit did not show significant difference in the healing rate(P>0.05). On day 6, as was similar to day 9, ecdysterone showed better efficacy in promoting wound healing than control and dexamethasone (P＜0.01), the latter having similar effect with the control management(P＞0.05),and that of ecdysterone was similar to Yunnan white power(P＞>0.05). Pathologically, early stage of wound healing was characterized by exudation and hyperemia, and on day 6 and 9, ecdysterone and Yunnan white power exhibited similar strong effect for shaping the granulation tissue and stimulating epithelial cell proliferation. Utraminiaturely, early stage of wound healing was characterized by disintegration in tissues and cells, and on day 6 and 9, ecdysterone and Yunnan white power exhibited similar strong effect for shaping blood capillary, collagenoblast and collagen fiber.ConclutionEcdysterone can obviously promote wound healing in rabbits, which may offer a clinical alternative for promoting wound healing. |
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