The Study Of Serum 8-Isoprostane, GSH-Px And Relationships With Insulin Function In Patients With Obstructive Sleep Apnea/hypopnea Syndrome

primary part The Investigation of Serum 8-Isoprostane, GSH-Px inPatients with Obstructive Sleep Apnea/hypopnea SyndromeObjective To investigate the expression of Serum 8-isoprostane, glutathioneperoxidase(GSH-Px) in patients with obstructive sleep apnea/hypopnea syndrome(OSAHS), and explore changes of oxidative stress in patients of OSAHS in vivo.Methods 15 patients with simple snoring and 65 patients with OSAHS wereinternalized in the study. After being collected common clinical data, all patientsunderwent a single:night polysomnogram to get polysomnogram variables. Serum8-isoprostane was measured by enzyme-linked immunosorbent assay (ELISA), and serumglutathione peroxidase(GSH-Px) was measured by spectrophotometer.Result 1. The levels of 8-isoprostane increase gradually with the increase of apneahypopnea index(AHI), while the levels of GSH-Px decrease gradually with the increase ofAHI. 2. 8-Isoprostane correlates with AHI, oxygen desaturation index(ODI), cumulativepercentage of sleep time with SaO₂ below 90% (CT90%), average SaO₂, minimum SaO₂,Epworth sleepiness scale(ESS), body mass index(BMI) respectively, meanwhile,correlation coefficient(r) decreased gradually from AHI to BMI. 3. GSH-Px also correlateswith ODI, AHI, average SaO2, CT90%, ESS, minimum SaO₂, BMI respectively, and rdecreased gradually from ODIto BMI.Conclusion 1. The severity of OSAHS is independently associated with oxidativestress. 2. Oxidative stress correlates with frequency of desaturation more strongly than with Second part The Study of Serum 8-Isoprostane, GSH-Px and InsulinFunction of Patients with Obstructive Sleep Apnea/hypopnea SyndromeObjective To explore the effect of oxidative stress and nocturnal intermittenthypoxemia in patients with obstructive sleep apnea/hypopnea syndrome(OSAHS) oninsulin resistance and function of islet beta Cell.Methods 15 patients with simple snoring and 65 patients with OSAHS wereinternalized in the study. After being collected common clinical data, all patientsunderwent a single-night polysomnogram to get polysomnogram variables. Serum8-isoprostane, fasting insulin(FINS) were measured by enzyme-linked immunosorbentassay(ELISA), and serum glutathione peroxidase(GSH-Px) was measured byspectrophotometer. HOMA-IR, FINS were used to estimate insulin resistance(IR),HOMA-βwas used to estimate islet beta Cell function.Result 1. With the increase of apnea hypopnea index(AHI), serum fasting bloodglocuse(FBG), FINS, 8-isoprostane, GSH-Px are significantly different among the snoringgroup, mild-moderate OSAHS group and severe group(P=0.0034, P＜0.0001), butHOMA-βdoes not differ significantly among the three groups(P＞O.05). 2. Serum FINSand HOMA-IR correlate with AHI, 8-isoprostane, GSH-Px, BMI respectively(P＜0.05).When adjusted for age and BMI with multiple linear regression analysis, FINS andHOMA-IR correlate with AHI, 8-isoprostane respectively as before. There are nocorrelation between HOMA-βand AHI, 8-isoprostane, GSH-Px, BMI(P＞0.05). 3. SerumFINS, HOMA-IR correlate with ODI, CT90%, minimum O₂, average SaO₂ respectively,with correlation coefficient(r) decreased gradually from ODI to average SaO₂ FINS andHOMA-IR correlate with ODI, CT90%, minimum SaO₂ respectively. When adjusted for age and BMI with multiple linear regression analysis, multiple correlation coefficient(R)decreased gradually from ODI, CT90% to minimum SaO2.Conclusion 1. Augmented oxidative stress and distinctive intermittent hypoxia ofOSAHS correlate with IR independently. 2. IR correlates with frequency of desaturationmore strongly than with severity of desaturation.