Primary Comparative Study Between Special Chromosomal Translocation-Associated And Nontranslocation-Associated Soft Tissue Sarcomas

Objective: To investigate the effects of arsenic trioxide (As2O3) in various concentrations on the proliferation and VEGF mRNA expression of HepG2 cells,and to identify the mechanism of putative signaling pathway in arsenic trioxide influencing VEGF mRNA expression.Methods: HepG2 cells cultured were divided into six groups:①negative control②different concentration of arsenic trioxide(0.1-7.0μmol/L)③A s2O3 +H7(block the PKC signaling pathway)④A s2O3 + PMA(acrive the PKC signaling pathway)⑤As2O3 and SB203580 (block the P38 signaling pathway)⑥A s2O3+SB203580 + H7. The optimal concentration of As2O3 was determined by the MTT analysis.The difference of VEGF mRNA expression in the three groups was detected by RT-PCR.Results:1)The absorbance of HepG2 cells was increased along with the increasing concentration of As2O3(from 0.1μmol/Lto1.0μmol/L)and the max-absorbance was detected 1.0μmol/L As2O3. The absorbance of HepG2 cells was descended along with the increasing concentration of As2O3(from2.0μmol/Lto7.0μmol/L)and the lowest-absorbance was detected 7.0μmol/L As2O3.2)VEGF mRNA expression was induced by different concentration of As2O3(1.0μmol/L,5.0μmol/L,7.0μmol/L),and 5.0μmol/L of As2O3 induced more VEGF mRNA expression.3)Comparing with control,the significant difference of VEGF mRNA expression was identified between the group of As2O3+H7 and As2O3+ SB203580, Comparing with As2O3,no significant difference of VEGF mRNA expression ; As2O3+H7+ SB203580 was opposite.Conclusion:①The effect of As2O3 in various concentrations was different:a low dose of As2O3 enhanced proliferation of HepG2 cells,and a high dose of As2O3 inhibited the proliferation of HepG2 cells.②As2O3 may induced the VEGF mRNA expression in HepG2 cells by the activition of PKC and/or P38 signal pathway.