| These years the study about the mechanism of liver injury and its prevention have became the the hot spot in biology and medical study in domestic and oversea. Kinetin is the main component of extracts of maize plumule(EMP), former experiments have already proved that EMP can boost up the stress reactionof drosophila, increase the appetite of drosophila and prolong life-span of drosophila. Experiments in silkworms have also proved EMP can advance the activities of SOD of silkworms, help skin cells of silkworms resist oxidation damage, protect the cell structure and protect channels. There aren't any articals about that have the protective functions in acute liver injury. This paper will use carbon tetrachloride to build the actue hepatic injury mice models and use the EMP to protect mice liver. and will descuse its working mechanism for the futher studying of protection of this illness and to find a effective method.This experiment will use CCl4 to build the experimental mice model and using EMP to protect the mice liver. Results showed that compared the model control group with normal control group, the serum ALT and AST increased significantly, the liver malonic aldehyde content increased significantly. The model control group liver tissue is turgid the centrol of the liver sub-phyllode showed spread and poly-incomplete necrosis and fat denaturation. The inflammation cells have been infiltrated. The mice acute hepatic injury experimential model was built very successfully.Compared the groups, who had lower and higher dosage(20,40 mg·Kg-1·d-1), with the model control group, we can see the serum ALT and AST decreased significantly (P<0.01,P<0.05) and the content of Alb increased significantly. EMP decreased the amount of MDA and NO and increased the activities of SOD,CAT and GSH-px of the hepatic injuried mice in liver tissue significantly. In the group with the higher amount of EMP, the serum ALT and AST decreased by 37.3% and 24.1%, the content of Alb increased by 25.7%, the activities of SOD,CAT and GSH-px of the hepatic injuried mice in liver tissue increased by 55.3%,42.1% and 62.5%, compared with the model control group, the difference is very significant(P<0.01). In the group with the lower and higher amount of EMP the hepatic sub-phyllode spread turgided, the fat denaturated, the liver cells of the centrol part were necrosised, less inflammation cell infiltration was noticed. Its function is the same with blfendate group.The results showed that EMP can resist acute liver injury in mice induced by CCl4. The mechanism of its antioxygenic properties may relate to its abilities of eliminating free radical, increasing the activities of antioxidative enzymes, and maintaining completeness of cell membrane.This experiment will use CCl4 to build the experimental mice acute primary hepatocye injury model and using EMP to protect the mice liver cells. Results showed that compared the model control group with the normal comtrol group, the culture medium ALT,AST and the malonic aldehyde content increased significantly. There are lots of cell fragment in the model control group, shrinking of cytoplasm and aggrandizing of intercellular space were also observed. After HE coloration, nuclear chromatin bordering, karyotheca splintering, volume intumescing, and vacuolar degeneration were obrserved under microscope. The mice acute primary hepatocye injury experimential model was built very successfully.Compared the groups, who had different dosage(40,20,10mg·L-1), with the model control group ,we can see the culture medium ALT and AST decreased significantly (P<0.01,P<0.05) and the content of Alb increased significantly. EMP decreased the amount of MDA and NO and increased the activities of SOD,CAT and GSH-px in culture medium significantly. In the group with the higher amount of EMP, the culture medium ALT and AST decreased by 35.1% and 35.3%, the content of MDA and NO decreased by 26.6% and 29.1%, the activities of SOD,CAT and GSH-px in culure medium of the injuried hepatic cells increased by 33.5%,44.6% and 42.7%, compared with the model control group, the difference is very significant(P<0.01). In the group with the different amount of EMP, the hepatic cell consistency was higher than model group, structure of liver cells was intact, less cell fragment was noticed. EMP can aslo increased the content of Bcl-2 and NF-κB in injuried cells significantly, the phenomenon of NF-κB shifting to nucleolus was noticed.The results showed that EMP can resist experimental mice acute primary hepatocye injury induced by CCl4. The mechanism of its antioxygenic properties may relate to its abilities of eliminating free radical, accelerating transcription of antioxidative enzymes, increasing the activities of antioxidative enzymes, maintaining completeness of cell membrane, restraining apoptosisand enhancing cell livability. |
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