The Study On Abzymes For Catalysed Selectivity Hydrolysis Of Ibuprofen Methyl Ester

Abzyme, or catalytic antibodies, is immunoglobulin that providedwith catalytic activities. It is a newly developed research area derivedfrom chemistry and biology. Because of highly selectivity and efficientcatalysis, to date, catalytic antibodies have been shown to catalyze a vastarray of different chemical processes, incorporating specificity, stereo-selectivity, and even the ability to route a reaction through a disfavoredchemical pathway. In fact, antibodies have now been uncovered forreactions for which there are no known natural or man-made enzymes. Itis great value both in theoretical and practical science. In this thesis, wecarry out to study on organic chemical reactions catalyzed by abzyme.Ibuprofen is antifebrile ease pain drug. Its s(+)-structure have biologyactivity. At present, we primarily use biology enzyme engineering topreparate s(+)- ibuprofen. However, abzymes for catalysed selectivityhydrolysis of ibuprofen methyl ester is not reported.This paper firstly develops the research of monoclonal antibody for catalyzing selectivity hydrolysis of ibuprofen methyl ester. According tothe transition state theory, we have simulated the structure of tetrahedronof ester hydrolysis to design and synthesize sulf-hapten and phosphorous-hapten and antigen. Using the object antigen for mice immunity, we havegained the monoclonal antibodies. Then, we compare these two kinds ofantibodies of hydrolysis activity and indicate that one monoclonalantibody can effectively catalyse race hydrolysis of ibuprofen methylester and the other can but catalyse hydrolysis of s(+)-ibuprofen methylester. In addition, sulf-hapten hydrolysis activity is more thanphosphorous-hapten.