Blood Compatibility Study Of Specific Immunoadsorbent In Myasthenia Gravis

Objective: To prepare a specific immunoadsorbent for pathogenic acetylcholine receptor antibodies (AChRAb) in myasthenia gravis (MG). The blood compatibility character of adsorbent was studied by whole blood perfusion extracorporeal circulation experiment. Methods: The cellulose beads were prepared as carriers. A 10 synthesized amino acids of main immunogenic region of human AChR or L-tryptophan, as ligands were immobilized on cellulose beads to make immunoadsorbents respectively. In this experiment the normal rabbits were randomly divided into three groups, including control group (only cellulose beads), specific group and L-tryptophan group. Blood cells, ions and plasma proteins were examined before and after experiment, t test was applied in analysis of the data. The rates of loss were compared by t test, too. Results: There was no significant difference in control group in blood cells, ions and proteins before and after experiment. There were non-significant differences between specific group and L-tryptophan group in blood cells and in ions, but plasma proteins were significant decreased. In plasma total proteins (TP) were 57.50±2.52 and 46.504±3.32 (P＜0.05). Plasma albumins (A1b) were 33.75±2.63 and 29.50±1.91(P＜0.05) and plasma globulins (Glo) were 23.75±1.89 and 16.75±2.36(P＜0.05) respectively in specific group. TP were 60.50±7.68 and 51.00±9.42(P＜0.05), Alb were 36.254±3.30 and 31.50±3.70(P＜0.05) and Glo were 24.25±4.57 and 19.504±6.40(P＜0.05) respectively in L-tryptophan group. The plasma proteins were non-significant in spec- ific group and L-tryptophan group (P＞0.05). Conclusion: The results were not influenced by control group. Blood cells and ions were not adsorbed obviously in specific group and in L-tryptophan group, but plasma proteins were non-specifically adsorbed. There were non-significant differences between specific group and L-tryptophan group in plasma proteins. The specific immunoadsorbent was favorable in blood compatibility character and it can be adapted to treat MG.