The Study On The Method For The Analysis Of Quinolones And Biomacromolecules By Fluoremitry And Its Application

The rapid development of analytical chemistry makes it breakthrough of a single subject area, and accomplish the transition from analytical chemistry to the analytical science covering many subjects such as chemistry and mathematics, physics, computer science, biology, and developing as a multidisciplinary subjects. One of the direction of development of analytical chemistry is to improve the sensitivity and selectivity of analysis, the another is to expand vigorously its space of development to life sciences field.Quinolones are a relatively new class of synthetic antibacterial agents and new drugs have continuously emerged. Currently its clinical application is increasingly widespreaded, so to establish high sensitive and selective detection of the drug molecule is imperative; The quantitative analysis of biomacromolecules such as nucleic acid and protein is the parameters of biological fluid measurement and genetic diagnosis. It is also the most active area in many subjects so as life science and chemistry. With the development of pharmaceutical and life science, the demand for the detection of drugs and biomacromolecules is becoming increasingly high, therefore people must use and develope more appropriate method to meet the requirements. Fluorimetry has been applied widely in pharmaceutical and biomacromolecule analysis because of its high sensitivity and good selectivity. In this paper, we have done the following work in the exploitation of new detection method of quinolones and biomacromolecules.Part 1 Study on the method for the analysis of the quinolones by the charge-transfer reaction. (1) The charge-transfer(CT)reaction between enoxacin as the electron donor and alizarin red as the electron acceptor has been studied by spectrophotometric method. The experiments indicated that in water solution enoxacin can form charge transfer complex with alizarin red, the composition ratio was 1:2,λ_max was at 524 nm, the absorbance was linearly related to the concentration of enoxacin in the range of 0～20 mg/L. The proposed method has been applied to the determination of enoxacin in tablets and capsules with satisfactory results. The recoveries of enoxacin were 99.4～100.5ï¼….(2) A new sensitive method for the determination of gatifloxacin(GFLX)has been developed based on the charge transfer reaction between gatifloxacin as the electron donor and chloranil(TCBQ)as the electron acceptor by fluorimetry. The method has been applied to the determination of GFLX in preparations and the result was same with spectrophotometric method. At the same time the charge transfer-synchronous fluorescence spectroscopy was proposed and used for the determination of GFLX in urine with satisfactory results.Part 2 Study on the method for the analysis of the biomacromolecules.(1) The characteristic of Resonance Rayleigh scattering(RRS)spectra of Iron(â…¢), deoxyribonucleic acid(DNA)and their ion-association complex have been studied in the optimum condition. The spectrum enhancement mechanisim and interaction force have been disccussed too. Based on that, a new simple method for the determination of DNA was proposed, and successfully applied to the determination of DNA in synthetic samples with the recovery of 93.3％～100.9ï¼…. Compared with the resonance Rayleigh scattering method of organic dyes, the present method has broad linear range and low detection limit.(2) The corresponding characteristics of the spectra, the influencing factors and the optimum conditions of the ion-association complex formed between Methyl Blue(MB)and protein in the presence of non-ionic surfactant Tween-20 were studied using Resonance Rayleigh scattering(RRS)technique. The spectrum enhancement mechanisim and interaction force have been disccussed too. And the method for the determination of protein was proposed and applied to the determination both in synthetic samples and bovine serum sample with satisfactory results.(3) The interaction between BSA and the inclusion complex ofβ-CD with DC was studied by fluorescence method. The result shows that in the pH10.9 NH₃·H₂O-NH₄Cl mediumβ-CD was interacted with DC to form 1:1 inclusion complex and resulted in the enhancement of the fluorescence intensity of DC, and the fluorescence intensity was enhanced further after the addition of the BSA. Based on that, using the inclusion complex as fluorescence probe, a new method for the determination of BSA was established in the first time and has been successfully applied to the determination of BSA in synthetic samples with satisfactory results.