Inhibition Of Sophorae Flavescentis On Acute And Persistent Infection In Vitro Induced By Coxsackie B3 Virus

Objective: Coxsackie B3 virus has been considered to be one of the mostcommon pathogens in human being because it can cause such diseases as myocarditis,cardiopericarditis and so on. Many studies have shown that anti-viral intervention canalleviate the clinical symptom. Astragalus has been already shown to be an effectiveagent against CVB3. In this article, we use astragalus as positive control to study thesophorae flavescentis extract's antiviral effects and its mechanism. Methods andresults: 1. we successfully established the model of persistent infection in vascularendothelial cells with CVB3. From the fifth passage, we used the supernatant ofCVB3-ECV304 cell to infect the Vero cell and detected the CVB3-RNA by RT-PCR.The results showed that supernatant could cause obvious CPE in Vero cell andCVB3-RNA could be detected. 2. In order to evaluate the sophorae flavescentis'santiviral effects, we classified the ECV304 cells into three groups and treat themdifferently. The antiviral effects were evaluated by MTT. In situ ELISA, cellprotection assay, RT-PCR and TNF-αdetection were employed to examine the changeof replication of CVB3 in persistent infection cell models. The results show that thesophorae flavescentis extract could inhibit the CVB3's pathogenical effect. Inpersistent infection model, the expression of CVB3 antigen was limited, the extent towhich CPE of Vero cell had been caused by supernatant of CVB3-ECV304 was foundto be decreased. The copies of CVB3-RNA and TNF-αtiter lowered. Conclusion: 1.All these assays can support idea that sophorae flavescentis can effectively inhibitboth acute and persistent infection of CVB3. 2. The mechanism of sophoraeflavescentis fighting against CVB3 seems to be similiar to that of astragalus, thus we can gain insight into the mechanism of sophorae flavescentis acording to astragalus'phannacodynamics.