Effects Of Gekko Sulfated Polysaccharide On The Differentiation Of Hepatic Cancer Cell Line SMMC-7721

Objects: To observe Gepsin on the proliferation and differentiation of hepatic cancercell line SMMC-7721. Methods: SMMC-7721 cells were cultured in RPM-1640medium supplemented with 10% fetal bovine serum at 37℃in a humidified 5% CO2incubator. SMMC-7721 cells were seeded in 24-well plates at 1×10~4 cells/ml. Cellswere allowed to grow for one day before being exposed to Gepsin(100μg/ml and 10μg/ml, respectively). SMMC-7721 cells were collected at 30min, then 24, 48, 72, 96,120, 144h after treatment with Gepsin. Trypan blue stain were used to determine totalcell count and viable cell number. SMMC-7721 cells were seeded in 24-well plates at1×10~4 cells/ml. Cells were allowed to grow for one day before being exposed toGepsin(100μg/ml and 10μg/ml, respectively), At day 7 after treatment with drugs,culture medium was harvested.Then AFP and ALB were detected byrayeroimmunology. At day 7after treatment with drugs, SMMC-7721 cells wereexamined by light microscopy. The level of VEGF, TGF-β1 supematant was detectedby ELISA. Results: Hepatocarcinoma cell line (SMMC-7721)were exposed toGepsin(100μg/ml and 10μg/ml). Gepsin strongly inhibited the proliferation ofhepatocarcinoma SMMC-7721 cells. While Gepsin did not have effect on viability ofSMMC-7721 cells. Treated with Gepsin, SMMC-7721 cells showed ultrastructuralfeatures of differentiation. AFP secretion decreased while ALB secretion increasedmarkedly on Gepsin-treated cells. SMMC-7721 cells showed a round shape underlight microscope, while cells changed to spindle shape after exposure to Gepsin. Thelevel of TGF-β1 significantly increased in Gepsin group (100μg/ml)(P＜0.01), thelevel of VEGF in suramin treated groups significantly increased than control group. Conclusion: (1)Gepsin strongly inhibited the proliferation of hepatocarcinomaSMMC-7721 cells. (2) The viability of SMMC-7721 cells was not suppressed byGepsin. (3)Gepsin could induce differentiation of SMMC-7721 cells.(4)Gepsin maybeinduce the differentiation of SMMC-7721 cells via TGF-β1 ways.