| Objective: Cerebral arterial thrombosis is a common and frequently encountered disease of nerve system, which has a rate of high death and disability. Most of the survivors complicated with dysgnosia and physical dysgnosia, so to bring a heavy burden for the family and society. The traditional Chinese medicine Huoxue Tongluo decoction which is created on the basis of traditional theory of Qi and blood and many years'practical experience has a protecting and curing function on cerebral arterial thrombosis. The curative effect was satisfactory proved by a great quantity of clinical cases. This study is to explore the mechanism of Huoxue Tuoluo decoction with serology method and radioimmunity method, so to provide theory evidence for its farther application in clinic.Methods: 50 healthy Wistar rats of male and female whose weight were 250-300g were chosen and divided into 5 groups,10 rats in every group: blank control group, model group, low dosage of Huoxue Tongluo decoction group (25g/kg)(small-dose group), high dosage of Huoxue Tongluo decoction group (50g/kg)(large-dose group), and nim group (10mg/kg). Low dosage group high dosage group and nim group were given the drugs and blank control group and model group given the same dosage of normal saline all by intragastric administration once a day and for 14 days.Then they were made to models except blank control group by common carotid artery shunt method. The rats were anesthetized with Ether to cut open their center necks and the left femoral veins were separated. Heparin (625u/ml) 0.3 ml was injected into the femoral vein, and tiny polyethylene vessel was inserted and connected with mini-apparatus infusionis. Polyethylene vessel home-made was inserted into right common carotid artery from abscopal part, then the vessel was clamped and connected with liquid reservoir. Left common carotid artery was clamped with tiny bulldog clamp, and the rats were exanguinated from the vessel in the right common carotid artery. The blood bled out was collected and retransfused from the left femoral vein. This period was the ischemia stage. Then bulldog clamp was lossened and blood bleeding from right common carotid artery was stopped. This period was reperfusion stage. All the rats in model group ,small-dose group,large-dose group and nim group were decapitated to get the blood after 20 minutes'ischemia and 24 hours'reperfusion, then blood plasma superoxide dismutase (SOD),malonaldehyde(MDA), endothelin (ET), calcitonin-gene- related peptide (CGRP) were determined. Wet weight of brain without olfactory bulb cerebellum and low-set brain stem was weighed to calculate the cerebral index. The brain tissue in the left side was gotten and weighted its wet weight, then it was dried to constant weight in 110℃drying oven for 24 hours to calculate brain water content. ET and CGRP in the brain in the right side were determined after homogenate.Results1 water content of brain and cerebral index1.1 water content of brainThe content obviously increased in model group compared with that in blank control group, the difference was significant (P<0.01). Compared with that in model group, the content obviously decreased in small-dose group,large-dose group and nim group, and the differences were all significant (P<0.05). There was no significant differences in water content among small-dose group,large-dose group and nim group (P >0.05).1.2 Cerebral indexThe index obviously increased in model group than that in blank control group (P<0.05). Compared with that in model group, the indexes obviously decreased in small-dose group, large-dose group and nim group(P<0.05). There was no significant difference in cerebral index among small-dose group, large-dose group and nim group(P>0.05). 2 Effect on blood plasma SOD and MDA2.1 Blood plasma SODThe content of SOD obviously decreased in model group than that in blank control group(P<0.01). Compared with that in model group, SOD increased in, large-dose group and nim group, the differences were significant(P<0.01). Compared with that in model group, SOD increased in small-dose group, the differences were significant(P<0.05).Compared with that in small-dose group, SOD increased in large-dose group , the differences were significant (P<0.05).2.2 Blood plasma MDAMDA obviously decreased in model group than that in blank control (P<0.01). Compared with that in model group, MDA increased in small-dose group, large-dose group and nim group, the differences were significant except that in small-dose group (P<0.05 ). There was no significant difference in MDA among small-dose group, large-dose group and nim group(P>0.05).3 Blood plasma ET and CGRP3.1 Blood plasma ETThe content of blood plasma ET obviously increased in model group than that in blank control group , the difference was significant (P<0.05). Compared with that in model group, ET decreased in small-dose group, large-dose group and nim group, the differences were significant (P<0.05). Compared with that in nim group, ET decreased in large-dose group, the differences were significant (P<0.05).3.2 Blood plasma CGRPThe content of CGRP obviously decreased in model group than that in blank control (P<0.05). Compared with that in model group, CGRP increased in small-dose group, and nim group, the differences were significant(P<0.05). Compared with that in model group CGRP increased in large-dose group(P<0.01). There was no significant difference in CGRP among small-dose group, large-dose group and nim group(P >0.05).4 ET and CGRP in brain tissue4.1 ET in brain tissueThe content of ET in brain tissue obviously increased in model group than that in blank control group, the difference was significant (P < 0.05). Compared with that in model group, ET decreased in large-dose group and small-dose group, the differences were significant (P < 0.05). Compared with that in model group, ET decreased in nim group, the differences were significant (P < 0.01).4.2 CGRP in brain tissueThe content of CGRP obviously decreased in model group than that in blank control group(P <0.05). Compared with that in model group, CGRP increased in small-dose group and nim group, the differences were significant (P <0.05). Compared with that in model group, CGRP increased in large-dose group the differences were significant (P<0.01).There was no significant difference in CGRP among small-dose group, large-dose group and nim group(P >0.05).Conclusion: The ability of clearing oxygen free radical of brain tissue is weakened, while its injury is strengthened after ischemic reperfusion of whole brain in the rats. Vaso-excitor materials like ET increase and vaso-relaxation materials like CGRP decrease, so to cause the contraction of blood vessel and edema of brain tissue. Huoxue Tongluo decoction can inhibit the production of lipid peroxide MDA, and relieve the injury of oxygen-derived free radicals after brain ischemic reperfusion. It also can rise up the activity of SOD, and improve blood circulation and edema in brain. |
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