The Establishment Of The Novel Method To Detect Break-fusion Events Related With AML1 Gene And Search Of Cases With New Partner Genes

Objective To establish the novel method to detect break-fusion events related with AML1 gene and search new partner genes.Method(1)The AML1/ETO fusion gene was detected in 87 acute leukaemia patients by reverse transcriptase-polymerase chain reaction(RT-PCR).(2)The mRNA expression of AML1 gene break-point region was screened in 87 acute leukaemia patients and 24 controls by a new semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR)and analysising with electrophoresis strip gray scale,using a constantly expression region of AML1 gene as internal reference.Result(1)The frequence of acute leukemia with AML1/ETO was 18.4%(16/87),and the frequence of AML-M2 and AML-M4 with AML1/ETO respectively were 39.47%(15/38)and 3.57%(1/29).None of the 24 controls was found to carry AML1/ETO fusion gene.(2)The mRNA expression of the break-point region of AML1 gene in patients with AML1/ETO fusion gene was significantly lower than that in control group(P＜0.001).The mRNA expression of AML1 gene break-point region decreased significantly in 31 acute leukemia patients and the frequence was 35.6%(31/87).(3)In these 31 csaes,the results of the two methods of 16 cases are coincident.We searched 15 cases which maybe carry new partner genes.These cases include 1 AML-M1 case,3 AML-M2 cases,7 AML-M4 cases,2 AML-M5 cases,1 AML-M6case and 1 ALL case.(4)The complete remission rates of doubtful case group and double negative group respectively were 50%and 53.3%.They both are lower then AML1/ETO positive group(78.6%).But there is no significant difference among these three groups complete remission rates(P＞0.05).Conclusion(1)The new method can detect efficiently more significant break-fusion events associated with AML1 gene.Combined with tradion RT-PCR means,the new method can be used as a efficient method which contribute to search its new partner genes.(2)The B/C method maybe contribute to judge the prognosis of acute leukemia.