Experimental Study On The Protective Effect Of Cerebral Ischemia Preconditioning On Brain Damage Induced By Subarachnoidal Hemorrhage In Rats

Objective: To explore the protection effect of cerebral ischemia preconditioning(IP) on brain damage induced by subarachnoidal hemorrhage(SAH) in rats.Methods: 81 Sprague-Dawley(SD) rats were randomly divided into six groups: group A(sham operation), group B(IP of 3min), group C(SAH control group), group D(IP of 3min...reperfusion for 24h...SAH), group E(IP of 3min...reperfusion for 72h...SAH), group F(SAH model group). "Modified four-vessel occlusion" method was used to replicate animal models of global cerebral ischemia and "autoblood injection into cisterna magna" method was used to replicate animal models of SAH. Neuroethology scores were detected in the 1,3 and 7days.Then the rats were decapitated in preset time and the brains were taken out. Arteria basilaris and Neuron in hippocampal CA1 region of rat were detected by HE staining. Ultrastructral change of arteria basilaris were observed by transmission electron microscope(TEM).Results: Neuroethology scores in group F were obviously higher and Neuron apoptosis in hippocampal CA1 region in it were obviously more than those of group A,B,C(p<0.05). it hinted that the animal models were succeed. Neuroethology scores in group D,E were obviously lower and Neuron apoptosis in hippocampal CA1 region in it were obviously less in comparison with group F(p<0.05).And group E had a significantly better effect than group D (p<0.05). Conclusion: 1 .Cerebral ischemia preconditioning(IP) had protective effect on brain damage induced by SAH. 2. Reperfusion for 72h after IP had a better protective effect than Reperfusion for 24h after IP on brain damage induced by SAH.