The Effect Of Plastrum Testudinis On The Expression Of BMP4 In Rats Of Parkinson's Disease

Parkinson's disease is the most common neurodegenerative disorder in the aged. Although the pathogenesis of Parkinson disease is concerned with genetic and environmental factors, the relative contributions are not very clear. The disease is because of degeneration in SN and the iter between the substantia nigra(SN) and striatum. The pathological changes1are the degeneration of dopaminergic neuron and the defect of DA in striatum because the DA's biosynthesis capability of the persistence nerve cells is breakdown. The clinical manifestation are myotone, static tremor, gait irregularity, decrease of emotional expression and so on.The pathomechanism of Parkinson disease is hepatic and renal yin deficiency while it is also contain heart and spleen in practitioner of Chinese medicine. Clinical manifestation are buffeting, rigor, brady action and so on.The reason of these clinical manifestation is because of on the foundation of deficiency in origin emerge the endogenous wind-evil and pyrophlegm which interact each other. The end-result lead to cacotrophia of muscle and vessel.Parkinson's disease most pertain to asthenia in origin and asthenia in superficiality while the origin are deficiency of both the liver and kidney and insufficiency of vital energy and blood, the superficiality are wind, fire, expectoration. The theory of practitioner of Chinese medicine considered that kidney is in charge of growth, bone and marrow forming. The traits of Plastrum Testudinis are saltytaste, sweet flavour, and calm. It has the efficacy of strengthening tendons and bones as well as nourishing YIN(feminine) and kidney. The treatment of Chinese crude drug in Parkinson's disease is lasting and efficacious, while the Western medicine most have adverse reaction. There are ascendancy in the treatment of Chinese crude drug.Objective:for the approach of the treatment adout plastrum testudinis, The breakthrough of this topic is BMP4. because BMP4 is one of the importment genome which can effect the neurodevelopment. In the different times, different genetic locus, BMPs have important effect. BMP4 is growth promoting, and an importment bone-forming and nerve-growth inducement factor. Hence, BMP4 would also have the important effect of recovery of nerve injury.Methods:From the molecular biology, the research examined the influence of Plastrum Testudinis liquor on BMP4mRNA, BMP4 and TH in SN and striatum through transfer and interpretation with the adoption of experiment technic of real-time fluorescence PCR, ELISA, Western-blotting etc.Conclusion:The research has accomplished the establishment of the Parkinson's disease rats we used the method which injected the 6-OHDA in SN and striatum, and the examination of BMP4's RT-PCR, and BMP4 ELISA. The result showed that BMP4 in the SN and striatum expressed low in normal, but it stepped up after the injured of SN and striatum, and then got down. While the expression of BMP4 in striatum on both gene level and protein level are at the equal pace which is different from the expression of BMP4 in SN. That would be because BMP4 is the important neurotrophic factor. After the injured of SN that leaded to the breakdown of BMP4, the paracrine of BMP4 from the other encephalic region cause its accrescence. The mechanism need deeper research.Under the instruction of "the kidney being in charge of development, marrow and bone", the research probed tentatively into the mechanism of yin nourishing and kidney replenishing medicine and the treatment of Plastrum Testudinis in PD rats. The research has accomplished the examination of BMP4's RT-PCR, BMP4 ELISA and Western Blotting of BMP4,TH,NSE. The result showed that BMP4 in the SN and striatum expressed an obvious increase, and dosage groups of seropharmacological Plastrum Testudinis are does-dependent. It provided that Plastrum Testudinis would treat through BMP4 to increase the expression of TH, and proved the treatment of Plastrum Testudinis in PD.