Research Of Effect On Fibroblast By Tripterygium And Extracts From Epiploon Adipose Tissue

Object:To found the model of fibroblast from bile scar tissue in vitro culture and study the influence of solution of tripterygium and extracts from epiploon adipose tissue to fibroblast throught the model and explore the effect of them to the formation of bile scar. Method: We cultivated fibroblasts derived from bile scar in vitro. Adding tripterygium or extracts from epiploon adipose tissue of different concentration into culture medium for fibroblasts. We evaluated cytokines in supernatant of epiploon adipose tissue , cellular apoptosis, fibroblasts proliferation and excretion and synthesis of fibroblast by flow cytometry , MTT assay, microscope observation. Results: In group fibroblasts growth was supressd , the supression got stronger with the increasing of the dosage and acting time of tripterygium; cellular apoptosis increased (P<0.05) ; cells proliferation reduced( P <0.05) . With the epiploon adipose tissue group, growth and proliferation of fibroblasts derived from biliary duct scar and normal biliary duct were promoted. Cytokines such as TNF-α,IL-2,IL-4,IL-10 were more in extracts than in blood (P<0.05) , Cellular apoptosis was not observed in this group. TGF-β₁ detected by ELISA was found that it increase significantly in epiploon adipose tissues. In Tripterygium experimental group, hypodiploid peak value of fibroblasts deteced by flowcytometry occured obviously. It suggested cellular apoptosis was promoted by Tripterygium. But in epiploon adipose tissue the same phenomenon was not found. It may suggest cellular apoptosis was restrained by epiploon adipose tissues. All of the experiments were detected in vitro culture system. Conclusion:Tripterygium can inhibite the growth of derived from biliary duct scar , and promote of it. Epiploon adipose tissue can excrete many kind of,these cytokines can promote collagen synthesis of fibroblasts, it may play a important role in the information of biliary duct scar.