High Through Put Screening Of Anti-liver Cancer Extrations Of Chinese Traditional Drugs And Study On LCID-1 Action Mechanisms

In the experiment, 1000 kinds of Chinese traditional drugs , a library of 20000 chinese triditional drug extraction was made use of the drug high throughput screening MTT method we had screened the library to get 3 drugs'IC50 about 10μg/mL.Take 20kg Chinese traditional drug material which contains activitic group to extract and purify further,until get their pure compounds .examined these pure compounds'bio-activity again ,we acquired a bio-activity pure compound LCID-1. I study the effect on human liver cancer cell line HepG2 was treated with LCID-1,get experiment results such as:1.The result of MTT showed, following treatment with LCID-1 under concentration of 0.01,0.1,1,10,100,1000μg/mL, the growth inhibition rate of liver cancer cell line was 6.15%,30.58%,52.72%,70.64%,96.26%,98.05%, respectively.Under the same conditions , the growth inhibition rate of the small rat normal liver cell were 5.92%,23.29%,41.94%,58.71%,81.59%,95.20% respectively.It shows that LCID-1 has less toxicity to human normal cells than to liver cancer cells.2.TUNEL result indicated 32.7﹪±4.8﹪tumor cells had the characteristics of apoptosis after LCID-1 treatment, while no apoptosis was found in HepG2 cells before treatment.3. RT-PCR indicated Bcl-2 and Ki67 mRNA over expressed in HepG2 cells in control, and the expression was downregulated after LCID-1 treatment .4. Immunohistochmistry staining showed overexpression of bcl-2 and Ki-67 in HepG2 cells was downregulated by LCID-1.The experimentation in vitro indicated:LCID-1,the Chinese traditional drug extraction has great merit of candidate drug to cure liver cancer.