| Objective: 1. To evaluate the expression of Bcl-2,Ki-67,P53,C-myc,Mdm2 andcyclin-D1 in malignant lymphoma (ML). 2. To study the relationship between theexpression of molecular index and prognosis for patients given autologoushematopoietic stem cell transplantation (AHSCT) for malignant lymphoma (ML).Methods: 1. Bcl-2,Ki-67,P53,C-myc,Mdm2 and cyclin-D1 were measuredby immunohistochemistry (IHC) on paraffin-embedded slices in 65 cases of MLand in 33 cases of ML who received AHSCT. 2. Survival analysis was done bythe Kaplan-Meier method and log-rank test. Multivariate analysis was carried outusing Cox proportional hazard model.Results:1. The expression of Mdm2, cyclin-D1 were negative. The positive rate of Bcl-2expression in B cell NHL was higher than that in T cell NHL (P＜0.05), but there wasno difference in the expression of Ki-67, P53, c-myc between two types of NHL.2. For 65 cases given regular treatment, the 3-year survival rate for patients withBcl-2(+) and Bcl-2(-) were 22.2％% and 55.56％, respectively (P＜0.05). But for Tcell NHL, there was no significant difference of survival time between the positiveand negative Bcl-2 group (3-year survival rate: 42.86％vs 61.54％, P＞0.05); 3-yearsurvival rate for ML patients with Ki-67(+) and Ki-67(-) were 43.75％and 85.71％,respectively (P＜0.05).3. For 33 patients with ML who received AHSCT, the 3-year disease free survival(DFS) of Bcl-2(+) group was 35.71％, while that Bcl-2(-) group was 88.89％. Therewas significant difference of DFS between the two groups. Meanwhile, for thesepatients, the 3-year DFS of Ki-67(+) and Ki-67(-) were 43.75％and 85.71％, respectively (P＜0.05).4. Multivariate analysis showed that the expression of Bcl-2 and Ki-67 were theindependent prognostic factors.Conclusion: 1. There was direct relationship between Bcl-2 protein expression andthe prognosis in B cell NHL. Combined examination of Bcl-2 and Ki-67 can beutilized to predict the prognosis of patients with B cell NHL. 2. The expression ofBcl-2 and Ki-67 were closely related with relapse after AHSCT in patients with ML.They were useful molecular makers for predicting the prognosis of patients with MLafter AHSCT. Objective: To investigate the relationship between the expression of 10-67, Bcl-2 andrelapse of Malignant Lymphoma paitents who had underwent the autologoushematopoietic stem cell transplantation (ASHCT) and routine chemotherapy.Method: Total 62 malignant lymphoma patients were divided into two groups. In thefirst group, 30 patients were given Autologous Stem Cell Transplantation. In thesecond group, another 32 patients were given the routine chemotherapy. All thesepatients' bone marrow and peripheral blood were obtained before ASHCT and routinechemotherapy, aiming to detected the expression of Ki-67 and Bcl-2 gene byimmunohistochemistry and Fluorescent Quantitation Polymerase Chain Reaction.Wilcoxon rank-sum test, Kaplan-Meier curve and log-rank were used to analyze therelation between the expression of ki-67, bcl-2 gene and relapse of these patients.Result: The expression of Ki-67, Bcl-2 gene has significant relation to the stage ofnon-hodgkin's lymphoma(NHL) and has no relation to the sex, age and Bsymptom.By immunohistochemistry, in the conventional group the positiveexpression rate of Ki-67 was 46.88％,the negative rate was 53.12％, the positiveexpression rate of Bcl-2 was 53.13％,the negative rate was 46.87％. In the ASHCTgroup, the positive expression rate of Ki-67 was 53.33％,the negative rate was 46.67％,the positive expression rate of bcl-2 was 43.33％,the negative rate was 56.67％. In theASHCT group, it has significant difference between the lower and higherKi-67ΔΔCT group in the bone marrow and peripheral blood, as same as bcl-2 group. Log-rank test indicate it has statistical difference between the overall survival rate oftwo groups(P=0.013).In the routine chemotherapy group, the expression of ki-67 andbcl-2 have the same result as the ASHCT group. The results for detection theexpression of Ki-67 and Bcl-2 in bone marrow and peripheral blood were consistentwith immunohistochemistry.Conclusion: It is effective method to detect the expression of Ki-67, Bcl-2 in the bonemarrow and peripheral blood by Fluorescent Quantitation Polymerase Chain Reaction.The expression of Ki-67 and Bcl-2 gene has significant relation to the relapse ofmalignant lymphoma patients after the ASHCT and routine chemotherapy. |
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