Study Of The Relationship Between Uteroglobin Gene Polymorphism And Henoch-Schonlein Purpura

Objectives To compare the differences between Henoch-Schonlein purpura (HSP) patients and normal control in respect of the UG gene polymorphism, and to investigate the relationship between the Uteroglobin(UG) gene polymorphism and the susceptibility, clinical type, pathological type of HSP and HSPN.Methods Totally 118 clinical-diagnosed Henoch-Schonlein purpura, including 80 Henoch-Schonlein purpura nephritis(HSPN) and 38 HSP without renal involvement were analyzed in this study. 100 normal healthy volunteers were selected as controls. Genomic DNA was isolated from peripheral blood leucocytes of all subjects. Exon1 of UG gene was amplified by polymerase chain reaction(PCR), and RFLP was utilized to detect the PCR products, then each genotype of parts of RFLP products was confirmed by direct sequencing. The distribution of UG gene polymorphism was compared in each group by means of case-control study, and the clinical and renal-pathological features of HSPN with different genotype were further analyzed.Results①Uteroglobin G38A genotypes and allele frequencies in Chinese Han children, were identical to the Japanese, Hungarian, Korean and Italy population (p＞0.05)②No genotype differences were observed for UG G38A polymorphism between HSP and normal control, HSP without renal involvement and normal control, HSPN and HSP without renal involvement, HSPN and normal control (p＞0.05).③No genotype differences were observed for UG G38A polymorphism between clinical phenotype of HSP and normal control (P＞0.05).④A significantly increased frequency of 38AA genotype of UG gene was found in HSP with increased serum IgE and HSP with normal serum IgE(58.82%VS8.43%,χ~2=21.946, P=0.000＜0.05, OR=15.51, 95%CI=4.93～48.84). The allele frequency of 38A was also significantly higher than 38G in HSP with increased serum IgE (67.66%VS32.53%,χ~2=14.698, P=0.000＜0.05, OR=4.68, 95%CI=2.05～9.18).⑤A significantly increased frequency of 38GG genotype of UG gene was found in HSPN with hypertension and HSPN without hypertension (75.68%VS18.60%,χ~2=26.172, P=0.000＜0.05, OR=13.61, 95%CI=5.01～37.01). The allele frequency of 38G is also significantly higher than 38A in HSPN with hypertension (85.14%VS50.00%,χ~2=21.960, P=0.000＜0.05, OR=5.73, 95%CI=2.76～11.88).⑥No association was observed between UG G38A polymorphism with gross hematuria, nephrotic syndrome, nephritic syndrome, haematuria, urine protein and renal pathological lesion of HSPN(P＞0.05).Conclusions①The uteroglobin gene G38A distribution in Chinese Han children were identical to the reported foreign populations.②Our results do not support a role for UG G38A polymorphism in susceptibility to development of HSP and HSP, and association with renal pathological lesion of HSPN.③The polymorphism of genotype of UG gene may be associated with increased serum IgE in HSP, the patients with 38AA genetype were susceptible to increased serum IgE.④The polymorphism of genotype of UG gene may be associated with hypertension in HSPN, the patients with 38GG genetype were susceptible to hypertension.