| The recent years research indicated that, is same with the entitytumor, the development and occurrence of the blood tumor also has close relationship witht he bloodvessel neogenesis and the signal conduction. At present this research gradually has become the hotspot which the multitudinous scholar pay attention to In the tumorforming process, the unusual bloodvessel neogenesis plays the key role. The tumor growth relies on the bloodvessel neogenesis. The tumor cell has potency which induces the bloodvessel neogenesis, under the stimulation of the growth factor, this kind of potency can transform into the phenotype which produce a marked effect.bFGF is one of the strongest bloodvessel growth factors, is one kind of extremely important regulating factor.The recent years research indicated that bFGF level is significantly higher than normal contrast in the leukemia patient body fluid, bone marrow has the phenomenon of the bloodvessel neogenesis. Each kind of experiment confirmed which in vivo and in vitro bFGF has activity of promoting blood vessel endothelial cell increment. The people discovered the leukemia cell has bFGF and/ or the bFGFR exceptionally expression. Thro-u gh autocrine or paracrine, bFGF can directly stimulates the leukemia cell, strengthening the tyrosine activating enzyme signal conduction circuit level to promote cell increment. The C-myc protein is an end differentiation negative adjustment factor, it has the vital role in the growth control,the differentiation development and cell apoptosis.Under the stimulating of the 2nd growth signal (crucial nature growth factor), the expression of C-myc nuclear factor can be adjusted thr- ough the tyrosine activating enzymesignal conduction circuit. So it can suppress the cell apoptosis and promoting cell increment, inducing the occurrence of tumor.The experiment groups of this research are 58 acute non- lymph leukemia (ANLL) patients who first diagnosis and 22 acute lymphleukemia (ALL) patient first diagnosis. The comparison groups are 40 ANLL completely alleviates (CR) patient, 4 ALL completely alleviates (CR)patients and 20 thrombocytopenic purpura (ITP)patients. We used RT-PCR method to detect the expression of bFGF and C-myc in the cell. The object is to study the inner link between bloodvessel neogenesis and signal conduction circuit.Thus we can have more profound understanding of acute leukemia, providing the new target spot for the acute leukmia treatment.Material and Method1,Experimental materialDrugs and reagent: MML-V, the RNA enzyme inhibitor, random primer, TAqenzyme, TAq enzyme, PBS fluid, dNTPs, 5×Buffer, 10×Buffer, indio primer, a-gar ose; Instrument: The low temperature centrifuge, The RT-PCR meter, The electrophoresis apparatus, ultraviolet transmissionreflection analyzer2,MethodThe research object is experiment groups are 58 ANLL first diagnosis patients, 22 ALL first diagnosis patients, the comparisongroup are 40 ANLL (CR) patients, 4 ALL (CR) patients, 20 thrombocytopenic purpura (ITP)patient. We Collect patient's bone marrow to separate single nuclear cell, withdraw cell total RNA and the DNA synthesis. According to the response condition of PCR response, we take 15u 1 amplification production through 2%agar sel gelatinelectricity, observing result and photographing. Using SPSS12.0 statistics software to carryon processing for the experimental result. Result1,The expression of bFGF and bFGFExpression of first diagnosis ANLL is significantly higher than CR, chi 2 examines the P value to be smaller than0.05, it has statistics remarkable difference. bFGF expression of first diagnosis ANLL is significantly higher than ITP, it has statistics remarkable difference, bFGF expression of first diagnosis ALL is significantly higher than CR, chi 2 examines the P value to be smaller than0.05, it has statistics remarkable difference. bFGF expression of first diagnosis ALL is significantly higher than ITP, it has statistics remarkable difference, bFGF expression of first diagnosis ALL is significantly higher than CR, chi 2 examines the P value to be smaller than0.05, it has statistics remarkable difference.2,The expression of C-mycC-myc expression of first diagnosis ANLL is significantly higher than CR, chi 2 examines the P value to be smaller than0.05, it has statistics remarkable difference. C-myc expression of first diagnosis ANLL is significantly higher than ITP, it has statistics remarkable difference. C-myc expression of first diagnosis ALL is significantly higher than CR, chi 2 examines the P value to be smaller than0.05, it has statistics remarkable difference. C-myc expression of first diagnosis ALL is significantly higher than ITP, it has statistics remarkable difference. C-myc expression of first diagnosis ALL is significantly higher than CR, chi 2 examines the P value to be smaller than0.05, it has statistics remarkable difference.DiscussionThe phenomenon of bloodvessel neogenesis in solid tumor has been generally approved by the people. Recent years people also have discovered the phenolmenon of bloodvessel neogenesis in leukemia. bFGF is a member of fibroblast growth factor(FGFs), one of the strongest bloodvessel growth factors. Gruber and so on reported than they had examined the high level bFGF of patient's blood serum and the marrow in hair cell leukemia (HCL), and it had biology activene ss. Exceptionally elevated bFGF may stimulate activator of plasminogen to release, producing fibringlysin.The fibringlysin can degrade extracellular matrix, taking part in the blood vessel neogenesis process.. Through the mechanism of autocrine/paracrine, bFGF can induce expression of VEGF in blood vessel endothelial cell, further confirming close relation between bFGF and tumor blood vessel neogenesis. This article examined bFGF expression of first diagnosis AL is higher than comparison group with RT-PCR method (P＜0.05), idicating AL has high expression of bFGF and has close relation with nosogenesis of AL.The expression dropout of C-myc gene has keyfunction in the nogogenesis of tumor. Some research already confirmed leukemia cell line HL-60cell has C-myc gene high expression. This experiment examines C-myc expression in first diagnosis AL is higher than comparison group and the ANLL expression example number (P＜0.05). Many research confirmed that nogogenesis of tumor has relationship with oncogene of growth factor information path. Growth factor binding to it's acceptor, it can induces activeness of tyrosine activating enzyme advance and activates the Ras-MAPK circuit,at last activating C-myc gene. This experiment examined bFGF and C-myc expression of first diagnosis AL is high, so we suppose that bFGF may adjust C-myc expression through tyrosine activating enzyme signal conduction circuit, inducing the nosogenesis of leukemia.Examination of bFGF and C-myc makes us to to realize that blood vessel neogenesis and the signal conductionat has key role in the nogogenesis of leukemia. Up to now, prognosis of leukemia bad, its first therapeutic tool is still bone marrow transplant and chemotherapy, the survival rate was low. Therefore, to aim directly at anti- bloodvessel neogenesis and inhibition of signal conduction will become the new way of treatment.Conclusion bFGF and C-myc have highe expression in acute leukemia, indicating they have close rlationthe with nosogenesis of AL, providing the the new target of leukemia treatment; bFGF and C-myc may have dependability... |
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