Synthesis Of Silver Carboxymethyl Chitosan And Study On Its Activity

Chitosan having most reserves in nature doesn't dissolve in water, which limits its application. Therefore the pop study way is to synthesize the new characteristic derivative of chitosan which could have the better water-solubility.Under the circumstances of reaction time, temperature and other conditions unchanged, changed the ratio between reagents, come to the optimum method of chloroacetic acid alkaline proportion of the reaction-chitosan : 30%NaOH to 1g : 120ml, NaOH : chloroacetic acid = 1:1.05 (mass ratio). 1g chitosan was modified to be finished product of 894mg by the method of alkalified chloroacetic acid, yield ratio was 98.4%. By the method of colloid titration, the degree of substitution of CMCTS(carboxymethyl chitosan) is 0.98, O-substitution : 0.78, N-substitution : 0.2.By the inspection adsorption process of CMCTS and silver ion, the results showed : CMCTS silver ion adsorption behavior accorded with quality role under the law and single molecular layer adsorption mechanism of kinetic equations advanced by Guoshun Zhang : t/Qt=t/Qeq+M/KCM(0) , adsorption capacity rest with the number of active groups, Q adsorption 2COOH replaced with the larger increases; In strongly acidic conditions (pH<2), 2COOH dissociation to be restrained on the silver ion complexation capacity greatly weakened, with the pH value increases, with silver ion complexation capacity increased gradually. when the pH reached 5~7, CMCTS Ag complex with the greatest degree of convergence. When the pH >7, first Ag+ and OH- form AgOH, then quickly emerged silver oxide precipitation, impacted measurement results. Assay results under different temperature showed that Q adsorption increased with temperature slightly increased, This indicates adsorption process is endothermic process, and warming is in favor of adsorption.The strong adsorption capacity to silver ion showed that CMCTS with the introduction of carboxyl, silver ion complexation capacity has been greatly enhanced, is about six times than chitosan, and is about 4.5 times than water-soluble oligomeric chitosan.At present, antibiotic and chemosynthesis druggery is commonly used, but it will destroy the microecosystem, bring hypersusceptibility. Therefore it is important to open out the efficiency druggery. In our study, we determine inhibition of CMCTS silver on . The activity results showed : CMCTS silver inhibition zone diameter become laeger with the increase of solution concentration, and When the concentration is at 40mg/ml, the inhibition zone diameter is 9.5 mm ( >9 mm), displayed preferable antibacterial effects. CMCTS silver minimum inhibitory concentration is at 1.25mg/ml. Animal experimental inflammation indicator analysis indicated: B3 drug group compared with periodontitis group, BI value presenced significant difference (P<0.05), PLI value is nearly significant (P= 0.07). Group C tinidazole, health group D, drug group B3 did not presenced significant difference, in the treatment of periodontitis CMCTS silver have certain effects, but cann't completely prevent the formation of dental plaque, and the therapeutic effect of CMCTS silver is inferior to finished products tinidazole.