Experimental Study On The Content Of Malondialdehyde And The Length Of Telomere In Liver Of Rat With Spleen Yin Deficiency Syndrome.

AbstractObjective: The purpose of this study was to observe the content of Malondialdehyde(MDA) and the length of telomere in liver of the rat model with spleen Yin deficiency syndrome, and further reveal the pathomechanisms of spleen Yin deficiency syndrome for providing a basis to study the practical pathology of it.Methods: First stage: The rat with spleen Qi deficiency syndrome was molded by the combined factors of overstrain and intemperance of taking food.Second stage: The rat with spleen Yin deficiency syndrome was established by a method of octo-hot damaged Yin drug. Two different methods of Thiobarbituric(TBA) acid and Southern-blot were used to determine the content of MDA and the length of telomere.Result: Compared with normal control, the content of MDA in liver of rat with spleen Yin deficiency syndrome was increased and the length of telomere was shortened.Conclusion: In the state of spleen Yin deficiency syndrome, its peroxidatic reaction was strengthen, and the DNA was attacked by the accumulation of free radical and those factors may result in shortening of the telomere. The experiment showed that the shortening of telomere was one of the pathomechanism of spleen Yin deficiency syndrome.