Research On The Quantitation Of D-antigen Of Poliomyelitis Sabin Strains And Detection Of Neutralization Antibody

Objective WHO points out that inactivated poliovirus vaccine is needed to maintain immune level to poliomyelitis after the realization of goal of eradicating poliomyelitis. More and more importance has been attached on Sabin inactivated poliovirus vaccine (Sabin IPV) because of its safety. The countries that are studying the inactivated vaccine from attenuated strain at present are China,Japan and Holland. Institute of Medical Biology has developed Sabin IPV and now they are doing clinical experiment. For IPV, its D-antigenic contents that concordance with its immunization efficiency in vivo is criticality target of IPV efficiency in vitro assay. At present, there is still short of standardized kit of Sabin IPV D-antigen quantitation in world. Our experiment aim at approaching and establishing a kind of method for accurate quantifying IPV D-antigen, and try to substitute micro neutralization analysis with the method to more quickly and more convenient monitoring antibody of poliomyelitis in crowd.Methods In order to study detect the D titers of IPV , Monoclonal antibodies against D-antigen of three types Sabin strain were used. We compared Indirect ELISA that use McAb with Indirect ELISA that use Polyclonal antibody and has been established by our laboratory for determining the Dantigen contents of trivalence IPV vaccine.We also detected the anti-poliovirus antibody in 80 human serums with Indirect ELISA that use McAb or Polyclonal antibody and micro neutralization analysis. Results we did not find significant difference between two detection results of IPV vaccine after by statistical treatment, and we also found there were not significant difference in Indirect ELISA that use McAb or Polyclonal antibody and micro neutralization analysis. Conclusion we considered that Indirect ELISA that use McAb or Polyclonal antibody may establish foundation of the efficacy detection normalization of Sabin IPV in vitro assay and also considered that we could establish a kind of substituent approach in monitoring antibody of poliomyelitis in crowd.