Effects Of Methylglyoxal On The Gene Expression Of VMP1 And ZNF510 In Human Periodontal Ligamnet Fibroblasts

Objectives: To further examine the differential gene expression of VMP1 and ZNF510 in human periodontal ligament fibroblasts (HPDLFs) induced by methylglyoxal (MG) through real-timePCR technology.Methods: HPDLFs were isolated from healthy periodontal ligaments of subjects who undergoes tooth extraction for orthodontic treatment. The tissue was mechanically scraped from the surface of extracted tooth and cells were prepared by using tissue explant culture technique. The cells were grown in DMEM medium supplemented with 15% calf serum. Experiments were performed with cells from the third to the fourth passages. Cells with density of 5×10~5/ml were devided into two groups ,one group was treated with 0.1mg/ml MG for 24h and another group was control group.24 hours later, Total RNA were extracted from the control group and the treated group respectively and were reversely transcripted into cDNA in time. The differential gene expression of VMP1 and ZNF510 in HPDLFs stimulated by MG was analyzed by using real-timePCR technology.Results:Real-timePCR analysis suggested that: VMPlgene was upregulated lightly and ZNF510 gene was downregulated after cells were treated with MG.Conclusions: MG has cytotoxic effects on HPDLFs. Furthermore, MG could alter the gene expression of VMP1and ZNF510 in HPDLFs. MG could be a factor which is related to periodontal diseases. The result of real-timePCR's analysis is almost consistent with the result of gene chip's analysis.