The Changes And Significance Of S-100β And Glial Fibrillary Acidic Protein In Serum And Cerebrospinal Fluid Of Epileptic Children

ObjectiveResearchers constantly pay much attention to the problem of brain damage induced by epileptic seizures. Various kinds of chemical materials in brain, such as enzymes,neurotransmitters,amino acids and so on, changed rapidly because of frequently on-going seizures and could lead to the death of brain cells.For the past few years,through the study of blood and cerebrospinal fluid(CSF), found that some biochemical markers could judge extent of neurologic damage and predict prognosis.These biochemical markers,including proteins, enzymes, neurotransmitters, cytokines, neuropeptides and so on, outweigh imaging detection in disease early stage . The roles of s-100βand glial fibrillary acidic protein(GFAP) as labelling proteins of astrocyte have attracted people's attention in brain damage. Our experimental purpose is to investigate the change of s-100βand GFAP in blood and CSF after seizure and their association,to determine the damage , extent and prognosis of brain damage and try to understand the role of astrocyte in epileptic seizures. Method1. Epilepsy group 41 cases, including generalized seizures 9 cases, partial seizures 20 cases, West syndrome 12 cases; control group 30 cases, were upper respiratory infection children who doubted intracranial infection on admission, after examination had been excluded .2. 24 hours after seizure,4 weeks,12 weeks after medicine,we taked venous blood 2ml,control group taked venous blood 2ml next day after admission,preserved supernate at -80℃after centrifugate; preserved CSF 2ml at -80℃after CSF examination for children who West syndrome.3. We detected specimen by double antibody sandwich enzyme-linked immunosorbentassy(ELISA).4. The data were dealt with SPSS11.0 statistical software by analysis of variance, t test and linear correlation.Results1. 24 hours after seizure, protein level of s-100β,GFAP in serum went up obviously, were significantly higher than those of control group(p<0.01); protein level of s-100β,GFAP in serum of West syndrome were higher than those of other groups(p<0.01); there were no significance between partial group and generalized group (p>0.05) .2. 24 hours after seizure, protein level of s-100β,GFAP in serum and CSF of West syndrome group went up obviously,there were highly positive correlation about protein level of s-100b,GFAP between serum and CSF.3. 4 weeks after medication, protein level of s-100β,GFAP in serum of epileptic group decresed ,but still higher than control group ,difference were significance(P<0.01).4. 12 weeks after medication, protein level of s-100β,GFAP in serum decreased obviously.According to epilepsy control criteria ,complete control 23 cases,excellence control 12 cases,effective control 4 cases,invalid 2 cases; Compared with other groups, invalid group higher significantly,there were no significance between other groups.5. Protein level of s-100βand GFAP in serum of epilepsy were highly positive correlation (r=0.54 ,p<0.01)。Conclusions 1. protein level of s-100β,GFAP in serum of epilepsy significantly higher than other groups; clinical control effect have association with protein rate of decay after medication. We can predict early brain damage ,degree of injure and prognosis2. For West syndrome ,protein level of s-100β,GFAP in serum and CSF are higher than those of other groups which illustrate severe brain injured and unfavorable prognosis.3. We may just take venous blood to evaluate brain damage because protein level of s-100β,GFAP are positive correlation in serum and CSF.but number of samples is small,so ,the result of experiment should further determine.4. Proteins of s-100β,GFAP are markers of glial cell,their changes illustrate gliocyte activation play an important role in epileptic brain damage.