The Relationship Analysis Of The Activity Changes Of AChE And BuChE In Whole Blood And Red Cells After Organophosphorus Pesticide Poisoning

Acute organophosphorus pesticide poisoning (OP) is a serious global threat to the human health nowadays, it's reported that the OP mortality rate ranges from 4% to 30% among different countries and regions, and the mortality rate of China is around 10%, much higher than that reported abroad.At present, the AChE government diagnostic classification standard of China is clinically employed to diagnose OP patients, most of whose butyryl cholinesterase (BuChE) activity changes in serum are simultaneously observed, but there hasn't be a universal government diagnostic classification standard made according to the BuChE activity changes in China. Then, is it right to employ the AChE poisoning diagnostic classification standard as the examination standard of the BuChE poisoning diagnostic classification? How is the relationship between AChE and BuChE? Whether BuChE activity changes could be employed as the poisoning diagnostic classification standard? In order to solve all the problems above, a series of experiments of healthy human blood and animals of organophosphorus pesticide intoxication in vitro are made to observe the activity changes of AChE and BuChE and to analyze the correlation of their activity changes, providing experimental evidence for the clinical diagnosis and treatment.Experimental results:1 Healthy human blood DDVP intoxication experiment in vitro: As the DDVP density decreased, both of its activity inhibitions against AChE in whole blood and red cells and BuChE in serum correspondingly weakened. BuChE possessed a lower activity than AChE, and they shared the approximately paralleled activity change tendency, displaying their linear regression relation (p <0.05). AChE activity, analyzed by t test, had no significant variance in whole blood and red cells with different densities of DDVP (p>0.05).2 Animal intoxication experiment: There were three animal intoxication experiments: the big rats DDVP intoxication experiment with four intoxication groups, the light group with no Atropine (DDVP 2mg/kg), the moderate group with no Atropine (DDVP 5mg/kg), the severe group with no Atropine (DDVP 7mg/kg) and the Atropine group; the big-ear albino rabbits intoxication experiment(DDVP 6mg/kg) and the big rats Rogor intoxication experiment (Rogor 4mg/kg). According to the experiment results, both of the activity inhibitions of AChE in whole blood and red cells and BuChE in serum decreased, and the decreasing level shared a linear relationship with the intoxication dosage on the whole, the BuChE activity possessed a more dominant decrease (p <0.05 ) , and AChE and BuChE showed the approximately paralleled activity change tendency. AChE activity had no significant variance in whole blood and red cells at different times in all the experiments (p>0.05).The experimental results suggest that AChE and BuChE share the paralleled activity change tendency after OP. The BuChE activity change couldnot reflect the OP level, but the inhibition of OP against BuChE is more dominant, if the BuChE activity in serum was employed as the clinical diagnostic criteria, one more adjusting coefficient should be added.