Study On The Signal Peptide Mediated Quantum Dots Tnrgeting In Living Cells

The plasma membrane is selectively permeable; it allows a very few molecules across it. The plasma membrane permeability greatly limited the application of most protein and peptide including some organic drug molecule, which can function as killing bacteria and virus. In recent years, it is found that some signal sequence of protein with biomacromolecular can penetrate the plasma membrane without energy or receptor. These sequences are called cell-penetrating peptide (CPP).Three biotinylated signal peptides (nuclear targeting sequences, NTS; endoplasmic reticulum targeting sequence, ETS;), was designed and synthesized by SPPS.The signal peptides were coated with avidin CdTe quantum dots (QDs). The purified QDs~NTS and QD~ETS were observed targeting in nuclear and endoplasmic reticulum separately.