| ObjectivesTo research the effects of antiendometrial antibody (EMAb) on pregnancy and its fetuses of rats. We aim to explore the pathogeneses and mechanisms of infertility and spontaneous abortion, in order to provide a guide of a clinical cure for them, as well as a clue of immunological contraception.Methods68 female rats were randomly divided into the following th(?)e groups. Group A (n=24), namely the low-dose experimental group, in which, the rats were injected pure human endometrial antigen (EMAg) with a dose of 90μg/kg into the abdominal cavity, and were injected the same dose again after a month. Group B (n=24), namely the high-dose experimental group, in which, the rats were injected EMAg with a dose of 180μg/kg into the abdominal cavity, and were injected the same dose again after a month. Group C(n=20),namely the control group, in which, the rats were injected 1ml 0.9% sodium chloride solution into the abdominal cavity, and were injected the same dose again after a month. For each group, 2ml blood was drawn from cervical venous sinus at the 6th and 8th weekend after the first injection of EMAg and centrifugalized respectively, and the serum was separated. Then EMAb and the levels of CA-125 in serum were detected by indirect enzyme-linked immunosorbentassay (ELISA), and electrochemiluminescence (ECL) respectively. After the second detection of EMAb, we choosed 2 rats with EMAb randomly in group A and group B and severed and reserved their uteri. Meantime, for the conception, the female and male rats were put together by the proportion of 3 to 1 for a week. Nonpregnant rats were killed and their uteri were severed and reserved in 4% formaldehyde solution. Pregnant rats were observed to whether miscarry or not during pregnancy. At the 21 st day of gestation, all pregnant rats were killed and their uteri were severed and reserved in 4% formaldehyde solution. Then we measured and compared the number, weight and length of newborn rats, the number of malformed newborn rats and the weight and diameter of placentae, and choosed randomly 2 placentae of each pregnant rat and reserved in 4% formaldehyde solution. Last, the reserved uteri and placentae were processed through the HE-dying technics, then we carried out histopathological examination and observed the histological structure of them through microscope. The data were processed statistically by a software of SPSS 13.0, and the positive rate of EMAb and the rate of infertility, abortion and malformation were showed as percentage, while the level of CA-125, the number, the weight and the length of newborn rats, and the weight and the diameter of placentae were showed as mean±SD(standard deviation). Multiple comparisons of all means were carried through Newman-Keuls test. There will be a significant difference if the probability is less than 0.05.Results1. The positive rate of EMAb: At the 6th weekend after the first injection of EMAg, the positive rate of EMAb of each group is respectively 16.67% (4/24, group A), 20.83% (5/24, group B), 18.75% (9/48, group A+B) and 0% (0/20, group C). There isn't a significant difference between each two groups (P>0.05).At the 8th weekend after the first injection of EMAg, the positive rate of EMAb of each group is respectively 20.83% (5/24, group A), 29.17% (7/24, group B), 25.00% (12/48, group A+B) and 0% (0/20, group C). There is a significant difference between group B and group C, and group A+B and group C (P<0.05), while there isn't a significant difference between group A and group B, and group A and group C (P>0.05).2. The level of CA-125: In the 6th weekend after the first injection of EMAg, the level of CA-125 of each group is respectively 25.58±0.06 U/ml (group A), 25.56+0.07 U/ml (group B), 25.57±0.08 U/ml (group A+B) and 25.55±0.10 U/ml (group C). There isn't a significant difference between each two groups (P>0.05).In the 8th weekend after the first injection of EMAg, the level of CA-125 of each group is respectively 25.56±0.05U/ml (group A), 25.57±0.04U/ml (group B), 25.56±0.03 U/ml (group A+B) and 25.56±0.04U/ml (group C). There isn't a significant difference between each two groups (P>0.05).3. The rate of infertility: The rate of infertility of each group is respectively 40.91% (9/22, group A), 59.09% (13/22, group B), 62.50% [5/8, group E (+)], 47.22% [17/36, group E (-)] and 15.00% (3/20, group C). There is an extremely significant difference between group B and group C (P<0.01), and there is a significant difference between group E (+) and group C, group E (-) and group C (P<0.05), while there isn't a significant difference between group A and group B, group A and group C , and group E (+) and group E (-) (P>0.05).4. The rate of abortion and the rate of malformation: During pregnancy, each group didn't miscarry, and all newborn rats weren't malformed. That is, both of the rate of abortion and the rate of malformation are zero.5. The number of newborn rats: The number of newborn rats of each group are respectively 10.77±1.79 (140/13, group A), 9.78±1.64 (88/9, group B), 9.67±1.53 [30/3, group E (+)], 10.32±2.14 [198/19, group E (-)] and 12.51±2.02 (213/17, group C). There is a significant difference between group A and group B, and all groups and group C (P<0.01), and there isn't a significant difference between group E (+) and group E (-) (P>0.05).6. The weight and the length of newborn rats: The weight of newborn rats of each group are respectively 5.47±0.42g (group A), 5.43±0.32g (group B), 5.33±0.24g [group E (+)], 5.48±0.41g group [E (-)] and 5.55±0.36g (group C). There is a significant difference between group E (+) and group C (P<0.05), and there isn't a significant difference between all of the other groups and group C, group A and group B, and group E (+) and group E (-) (P>0.05).The length of newborn rats of each group are respectively 6.40±0.19cm (group A), 6.39±0.23cm (group B), 6.37±0.21cm [group E (+)], 6.39±0.20cm [group E(-)] and 6.42±0.18cm (group C). There isn't a significant difference each two groups (P>0.05).7. The weight and the diameter of placentae: The weight of placentae of each group are respectively 0.67±0.06g (group A), 0.71±0.08g (group B), 0.74±0.05g [group E (+)], 0.70±0.09g [group [E (-)] and 0.65±0.07g (group C). There is a significant difference between group A and group C, group E (+) and group E (-) (P<0.05), while there is an extremely significant difference between group A and group B, group B and group C, group E (+) and group C, and group E (-) and group C (P<0.01).The diameter of placentae of each group are respectively 14.92±0.84mm (group A), 15.31±1.32mm (group B), 15.48±1.20mm [group E (+)], 15.39±1.31mm [group E(-)] and 14.69±0.73mm (group C). There is a significant difference between group A and group C (P<0.05), and there is an extremely significant difference between group A and group B, group B and group C, group E (+) and group C, and group E (-) and group C (P<0.01), while there isn't a significant difference between group E (+) and group E (-) (P>0.05).8. The results of histopathological examination: In the nonpregnant uteri, the glandular cells of the endometrium arrange irregularly, and have many small bubbles in them of the experimental groups with EMAb, while the glandular cells arrange regularly, and have few small bubbles in them of the control group.In the pregnant uteri of the experimental and control groups, their structure hasn't a significant difference. The glands of the endometrium proliferate highly, and form papillae or cauliflowers projecting into the uterine cavity. The glandular cells of the endometrium are highly columnar and arrange regularly.In the placentae of the experimental and control groups, their structure hasn't a significant difference, and possess also abundant blood supply.Conclusions1. Pure human endometrial antigen (EMAg) injected into the abdominal cavity can induce the production of antiendometrial antibody (EMAb) in the serum of the rats, and the positive rate of EMAb isn't related to the injected dose of EMAg.2. EMAb can decline the infertility rate of rats, and result in the decrease of the number and the weight of newborn rats.3. EMAb can make the weight and diameter of the placentae increase, and the increasing quantity is related to the injected dose of EMAg, that is, it increases more highly in the high dose group (180μg/kg) than in the low dose group (90μg/kg) . |
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