The Changes Of Epileptic Rat's Spatial Learning Memory Ethology And Immunoreactivity Of C-Jun And GABA In Cingulate Gyrus

Background: Epilepsy is common disease of central nervous system. Its principle feature is repeating seizures. The cognitive disturbance that epilepsy results in has brought great suffering to the patients and his family. In the past, the investigation about the cognitive disturbance of epilepsy had been mainly on the hippocampus. However, the investigation on cingulate gyrus was relatively less. Account of the important position of cingulate gyrus on the formation of memory and seizure of epilepsy, it is deserved to investigate the correlation between cingulate gyrus and the cognitive disturbance of epilepsy. Identifying this problem will improve our knowledge about the cognitive disturbance of epilepsy and provide a new way to treat epilepsy patients.Objective: To observe the ethology features of epilepsy rat during the model building of epilepsy; To observe the ethology features of spatial learning memory that epilepsy rat emerges in the Morris Water Maze; To investigate the immunoreactivity of C-Jun and GABA in the cingulate gyrus and the correlation between the cingulate gyrus and the epilepsy rat's spatial learning memory.Method: In this experience, we applied healthy male adult Sprague-Dawley(SD) white rats to make epilepsy models by injecting kainic acid and to make epilepsy models that are not intractable by using kainic acid in cooperation with scorpion venom. We observed the changes of animal models'spatial learning memory by the Morris Water Maze and theimmunoreactivity of GABA and c-jun in the cingulate gyrus by immunohistochemistry.Results: (1) 50 SD rats were used to make animal models, among them, 12 became intractable epilepsy model and 7 became epilepsy model that are not intractable; (2) Compared with the blank, intractable epilepsy model appeared obviously decreased spatial learning memory in Morris Water Maze, however, epilepsy models that are not intractable appeared no obvious differences; (3) The immunoreactivity of GABA and c-jun in the cingulate gyrus of intractable epilepsy model was decreased obviously compared with the blank. However, the epilepsy model that are not intractable appear no obvious differences. Moreover, in the cingulate gyrus, there was an obvious correlation on immunoreactivity between GABA and c-jun.Conclusion: (1) The key point of epilepsy rat's spatial learning memory impairment is if the epilepsy have become to intractable epilepsy; (2) The immunoreactivity of GABA and c-jun in cingulate gyrus of intractable epilepsy rat decreases obviously and there is an obvious correlation on the changes of immunoreactivity between GABA and c-jun; (3) The impairment of spatial learning memory that intractable epilepsy results in is correlated to the damage of GABA energy neuron in cingulate gyrus.