Expression Of Apoptosis Factor Bax And Nitric Oxide Synthase(NOS) In The Lingual Mucous Membrane Of The Diabetic Rat Model

Objective: In this study, compared the expression of apoptosis factor bax and NOS in the lingual mucous membrane of the diabetic rat model with the normal rat, and summarized the effects of bax and NOS when diabetes happens on cells of lingual mucous membrane.Methods: Injected to low dose streptozotocin to the rat which were fed with high-energy animal feeds, and then prepare the model of diabetic rat. Dyed the tissue slice by HE, and researched the thickness of lingual mucous membrane. Dyed the frozen slice of the tongue with SABC that belonged to the method of immuno-histochemistry, and measured the expression of bax in the lingual mucous membrane. Dyed the frozen slice of the tongue with NADPH-d that belonged to the method of histochemistry, and measured the expression of NOS in the lingual mucous membrane. Meanwhile analyzed the data with the processing system of image analysis.Results: Firstly, compared to the normal control group, the thickness of lingual mucous membrane, the thickness of the cuticularlayer , the number of the cuticularlayer , the height of tongue papillae and the basal cell density of tongue papillae of experimental group decreased (P<0.05). Secondly, compared to the normal control group, the expression of bax in the lingual mucous membrane of the experimental group was higher (P<0.05). Thirdly, compared to the normal control group, the expression of NOS in the lingual...