| Objective: To observe the pathological changes in acute lung injury (ALI) induced by intratracheal administration of oleic acid (OA) to rat and the effect of the intervention by macrolides on oleic acid induced ALI for rat. To compare the level of TNF-α and IL-10 in lung tissure / serum in OA model group and macrolide pretreat/posttreat groups. To investigate the intracellular mechanism of anti-inflammatory and immuno regulation effects of macrolides on ALI or systemic inflammatory response syndrome(SIRS). Methods: 48 wistar rats were randomly divided into normal control group given saline(0.15ml/kg) from tail vein, ALI model group injecting OA (0.15ml/kg) from tail vein and pretreat/ posttreat groups with injecting EM(0.75mg/kg) or AZM (0.15mg/kg) from tail vein also. 4h before or after establishing ALI rat models, then observered the pathological changes and wet weight/dry weight (W/D) of lung, tested TNF-α and IL-10 in lung tissure and serum. Results: compared with normal group, the ALI model group had the significant changes in lung W/D, pathology score and the level of TNF-α and IL-10 both in lung tissure and serum. There were more inhibitation in pretreat/ posttreat groups for all over the pathological damage than that of ALI model group (P<0.01 or P<0.05). Conclusion: Macrolides have a limited protection on oleic acid-induced acute lung injury in rat by inhibiting the inflammatory reaction. |
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