The Effect Of VEGF-C On Invasive Capability And Proliferation Of Human Cholangiocarcinoma Cells

Objective To investigate the effect of vascular endothelial growth factor-c (VEGF-C) on invasive capability and proliferation of human cholangiocarcinoma cells.Methods The human cholangiocarcinoma cells (FRH-0201) were cultured and stimulated with various VEGF-C(1ng/ml, 5ng/ml,10ng/ml ,50ng/ml ,100ng/ml ) , serum-free RPMI-1640 medium as control. The cells were cultured for 24,48 or 72 houres. The effect of VEGF-C on FRH-0201 was assayed by MTT. The cells stimulated with VEGF-C of various dose (1ng/ml,5ng/ml,50ng/ml) were made into 1×10~6 /ml cell hangs, The cycle pattern and apoptosis was assayed by using flow cytometry. The effect of VEGF-C on homotypic adhesion and metastasis in FRH-0201 was examined with ~3H-TdR infiltration and Boyden chamber.Results VEGF-C could enhance the proliferation of FRH-0201 in a dose and time dependent manner. And VEGF-C could inhibit cell apoptosis significantly. VEGF-C of 1ng/ml and 5ng/ml could enhance the proliferation of FRH-0201 obviously and the ability of VEGF-C over 10ng/ml dose gradually slowed down and there were no suppression phenomenon within 100ng/ml. After cultured for 2 hours with 1ng/ml,5ng/ml,10ng/ml of VEGF-C, the cells in the lower chamber were remarkablly more than the control group. After 60 , 90,120 minutes induction by 1ng/ml,5ng/ml, 10ng/ml,the homotypic adhesion of the cells showed significantly lower than that of the control group. Conclusion VEGF-C could enhance the proliferation of FRH-0201 and inhibit cell apoptosis. VEGF-C could decrease homotypic adhesion of FRH-0201 and may be a cause of the metastasis.