| Objective:To observe the inhibition of iodide on D-Galactose-induced cataractous lenses of rats.Methods:40 Wistar rats ,which are 4 to 5 weeks old and between 55 and 65 gram, were randomly divided into four groups(10rats/group): Normal Control,Model,Protective,Iodide groups.50% D-Galactose (30ml/kg) was administered daily by twice intraperitoneal injections, and there should be four hours interval between them, in two weeks to reproduce cataract of rats in the model group. Sodium Chloride(30ml/kg) was administered daily by twice intraperitoneal injections, and there should be four hours interval between them, in three weeks in the group of normal control. In the protective group and the iodide group, iodide (1.5nmol/g) was administered by intraperitoneal injection for three weeks, but in the protective group, 50% D-Galactose was administered daily by intraperitoneal injection from the second week as the model group. At day 11,14,17,21 the transparency of all lenses were observed through slit lamp microscopy. After 3 weeks, the levels of Superoxide dismutase(SOD) and Maleic Dialdehyde(MDA) were measured respectively.Results:All lenses were transparent in the control group and the iodide group. The lens opacity of the protective group rats were not so obvious as those of the model group rats at the day 11,14,17,21.The levels of SOD in the model group were obviously lower than that in the normal control,the protective and the iodide groups(P<0.01).The levels of MDA in the model group were obviously higher than that in the normal control,the iodide and the protective groups(P<0.01).Conclusion:The method of 50% D-Galactose (30ml/kg) being administereddaily by twice intraperitoneal injections can successfully reproduce D-Galactose-induced cataractous lenses of rats. Iodide could protect lenses of rats against oxidative damage by increasing activities of SOD and inhibit or delay the generation and development of galactose- induced cataract at the early stage. |
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