| Objective: To establish SMMC-7721transplantation tumor model in Balb/c athymic mouse. And investigate the Histopathology change and the restrained growth of SMMC-7721 hepatic cancer planted in athymic mouse after low frequency ultrasound radiate microbubble; the local concentration of local free-radicle and the expression of CD105 was observed, then explore the mechanism of this action.Methods: Adjust SMMC-7721 cell concentration to 5×106/ml, then subcutaneousely planted into balb/c mice for establishing models, 0.2ml evey mouse. After the model established ,fifty mice were randomly divided into five groups, control group(A), simplicity microbubble group (B), simplicity ultrasound(C), ultrasound and microbubble qd group (D) and ultrasound and microbubble qod group (E). After disposal correspondly, measure the volum of gross tumor for a week, Then, draw a growth curve and count the ratio of tumor growth, collect the tumor sample and histopathology damage was observed. The local concentration of local free-radicle was observed by immunohistochemistry with anti-SOD. relation between tumor growth ratio and concentration was analyzed.And observe the CD105 concentration in immunohistochemistry was observed with anti-CD105, then calculate the MVD of each group and analyz the relation between MVD and tumor growth ratio.Results: Two weeks later, the model established. After disposal correspondly for a week, tumor volume of each group has obviously difference(cm3) , the volume of group D(0.139±0.029) and group E(0.122±0.025) were significantly smaller than those of group A(0.207±0.081), (P<0.05). No statistics difference among groupA,B and C,so dose between group D and E. Statistical difference were been between group A(121.24±0.347)and group D(73.20±0.138) or E(57.24±0.1653)in ratio of volume growth(%), (P<0.01). Much thrombogenesis and infarctus can be observed in histopathology in group D and group E Concentration of SOD (%)in tumor tissue of group D (28.83±2.483)and E(32.50±3.017)... |
PDF Full Text Request