| Objective: The aim of this study was to investigate the neuroprotective effect and mechanism of ASICs blocker on ischemia-reperfusion injury in rats. Methods: The model of focal cerebral ischemia-reperfusion in Sprague-Dawley (SD) rats was established with sutured-occluded method invented by Zea Longa. Sixty-four SD rats were randomly divided into four groups including sham operated group, focal cerebral ischemia-reperfusion model group, amiloride â… group (repetitive administration two times) and amiloride â…¡ group (repetitive administration five times), and each group was sixteen rats. First, the eight rats in each group were randomly taken for the evaluation of neurological deficit score, the measurement of cerebral infarct volume and HE stain of brain tissue at twenty-four hours reperfusion after two hours focal brain ischemia. Second, the other eight rats in each group were taken for determination to contents of myeloperoxidase (MPO) and nitric oxide (NO) from the brain tissue homogenate by biochemical reagents at twenty-four hours reperfusion after two hours focal brain ischemia under the same model. Results: (1)Compared with amiloride â… and amiloride â…¡ group, the scores of neurological deficit and brain infarct volume were significantly higher in the focal cerebral... |
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