Study On Correlation Between The Expression Of Neuronal Nitric Oxide Synthase And Pathological Morphologic Changes Of Muscle Fiber In Foot Muscles Of Congenital Clubfoot

Objective To study the expression and position of neuronal nitric oxide synthase(nNOS) and pathological change of muscle fiber in foot muscle of congenital clubfoot (CCF), analyze whether there exists a regular correlation between nNOS and cross section area(CSA) of skeletal muscle fiber, In order to explore the mechanism and significance of nNOS in neuro-muscular etiological theory of CCF and indicate the clinical therapy.Methods We study twenty patients of stiff CCF aged from 6 months to 3 years (mean 18months) requiring surgical treatment. there were twelve boys and eight girls. Muscle specimens of anterior tibial , peroneus brevis, post tibial and gastrocnemius from patients were obtained in the course of corrective orthopaedic surgery of CCF. Foot muscle samples of normal control group from 5 injured children aged 1 to 3 years without neuro-muscular disease in operation. All the specimens were examined using light microscopy and hematoxylin and eosin and anti-nNOS immunohistochemistry staining. We observed the distribution of nNOS positive staining region and pathological change of muscle fiber.Quantitative measurement of the anti-nNOS staining and the CSA of skeletal muscle fiber were performed for statistic analysis and study on association between the expression of nNOS and CSA of skeletal muscle fiber. Results (1)The expression of anti-nNOS immunohistochemistry staining...