The Investigation Of Rats's Kidney Morphological Change And Apoptotic Mechanisms By HSP70 Induced By High Temperature After Scald

Objective: To explore the protection of HSP70 induced by high temperature on thescalded Wistar rats' kidneys.Methods: Wistar rats were randomly divided into Normal control group (GroupN, n=6), scalded group (Group B, n=18) and shock induced group (Group S, n=18).The models of rats with 30% third-degree scalds and rats with 30% third-degreescalds after HSP70 generations induced by hyperthermia were made, and HEmethod, immunohistochemical technique and stereological method were used toexplore the morphological change of kidney in Group N and other two groups at6h,12h,24h after scalds and the location and quantity of HSP70 and Caspase-3 inkidney.Results:1. The normal kidneys were fabiform or Horseshoe shape, and the surfaces were smooth and ruddy. The volumes were (0.5900±0.0405) cm~3. But the kidneys of every time point after scalds were intumescent, cardinal red and hyperaemic [(0.7183±0.0264)cm~3,(0.7200±0.0316) cm~3,(0.7217±0.0331) cm~3]. There was significant difference between the normal group and the scalded group (P<0.01) . There was no significant difference between the Group S [(0.6450±0.0476) cm~3,(0.6367±0.0516)cm~3, (0.6500±0.0316)cm~3]and the normal group (P>0.05), but there was significant difference between Group B and the Group S (P<0.05 ) .2. There was much change in kidneys to some extent at every time point after scalds. Renal glomerulus were contracted and ischemic, and capsular space became large. The pathological change of renal tubule was cloudy swelling and dropsy. Lumens were expanded. The change in the scalded group was more serious than that in the Group S.