Distribution Of Aminoglycosides Modifying Enzymes And Methyltransferases Genotypes In Imipenem-resistant Pseudomonas Aeruginosa

Objective Pseudomonas aeruginosa (PA) is the primary pathogenic microorganism of nosocomial infections. PA is resistant to many kinds of antimicrobials. It is very hard to treat the patients with PA infections. The mortality is high. Aminoglycosides are a vital component of antipseudomonal chemotherapy implicated in the treatment of variety of infections. These agents are bactericidal and exhibit synergy with other antimicrobials, most notably β-lactams, with which they are often administered for the treatment of PA. The resistance of PA to aminoglycosides has been found since 1960s and now becomes a global problem. The aims of this study are to reveal the susceptibility of imipenem resistant PA (IRPA) to six kinds of aminoglycosides (amikacin, gentamicin, isepamicin, tobramicin, kanamycin and netilmicin) and to describe and compare the distributions of aminoglycoside modifying enzymes (AMEs) and methyltransferases.Methods We collected 146 strains of IRPA from five area of China (Chengdu, Hangzhou, Beijing, Shanghai and Guangzhou). The agar dilution method was used to detect the minimum inhibitory concentrations (MICs) of IRPA to amikacin, gentamicin, isepamicin, tobramicin, kanamycin and netilmicin. PCR were used to amplify the genes of AMEs and...