The Effects On NGF Expression After Coadministration Of Bone Marrow Cells With Bcl-2 To Cerebral I/R Injured Rats Via Internal Carotid Artery

PrefaceBone marrow stromal cell (BMSC) is a source of neuro - tranplantation. BMSCs can differentiate into neurous and release of trophic factors such as NGF, GDNF. Many experiements have proved that BMSCs is effective on improvement of neurological functional deficits after stroke in rats.Bcl - 2 gene is the first gene that has been acknowaged to have the function of suppress eukaryon cell apoptosis. Bcl -2 is also effective on improvement of neurological functional deficits after stroke in rats.NGF is the most important tyophic factors that facilitate repair of damage brain. The expression of NGF can be improved if the brain was damaged.Experiments maded by Yi Li, Chen et al has proved that coadministration of BMSCs with a cell permeable inhibitor of Caspases, Z - Val - Ala - b - Asp -fluovomethylketone (Z - VAD) , into the MCAo rats promotes BMSCs survival and improve cutcome. We tested whether Coadministration of BMSCs with bcl -2 into the MCAO rats improves functional recovery after stroke and observe NGF exoression.Materids and Methods40 adult male wistar rats were assigned randomly into 4 groups: nothing group (n = 10) , BMSCs group (n = 10) , bcl -2 group(n = 10) and BMSCs with bcl -2 group (n =10). Then each group was divided into 2 subgroups;3 days, 14 days, according to the time after reperfusion onset. Thereafter, middle...