Construction Of Herpes Simplex Virus Ⅱ CTL Epitope GD DNA Vaccine And Study Of Its Immunological Characteristics

Genital herpes, a common sexually transmitted disease, is primary caused by h herpes simplex virus type 2 (HSV-2). The incident of GH has greatly increased in China recent years. Following primary infection at skin or mucosal surfaces, viral DNA is rapidly transported to the innervating ganglia by retrograde axonal transport. Here the viral genome circularizes and establishes a life-long latency in sensory neurons. There are no any anti-virus drugs, which can effectively control the occurrence and recrudescence of HSV-2. DNA vaccine, which can fully induce immune response, is the one of most optimising vaccines against HSV-2.In this research, we constructed a recombinant plasmid composed of two truncated regions of HSV-2 gD protein and gB CTL (cytotoxic T lymphocyte) epitope. To study the expression of this recombinant plasmid pVAX-gD-CTL in Mammalian cells COS-7. To evaluate the immunological characteristics of it, especially on cellular immunity.PCR primers were designed according to DNA sequence of HSV-2 glycoprotein D (gD) offered by HSV-2 333 strain on Gene Bank. The gD gene fragment was amplified and ligated into the eucaryotic expression vector pVAX1. After corresponding enzyme treatment, insert gB protein coding-CTL epitope. These...