Expression Of Bax/Bcl-2 In IL-10 Treated Hepatic Fibrosis Rat

ObjectiveTo study the expression of Bax/Bcl-2 in rat hepatic fibrosis and study theantifibrogenic effect of IL-10.Methods47 SD rats were randomly divided into two groups, normal control group (GN, 6 rats) and CCl_ group (GC, 41 rats) .At the end of 9th week, rats in GC were randomly allocated into three groups: model group (GM, 9rats), IL-10 treated group (GT, 9 rats) and recovered group (GR, 9rats) .Rats in GM were sacrificed at the end of 9th week and rats in GT and GR were sacrificed at 12th week.Reverse-transcription polymerase chain reaction (RT-PCR) was used to analyze Bax/Bcl-2 mRNA from freshly isolated cells. Immunohistochemistry was performed to detect Bax/Bcl-2 protein expression in liver tissue.ResultsRat hepatic fibrosis was developed as showed by histological examination, the degree of hepatic fibrosis in GT alleviated obviously compared to GM, and the change in GR was not distinct. Both immunohistochemistry and RT-PCR showed : the expression of Bax was increased clearly in GM (P<0.01) ; and Bcl-2 expression was also upregulated ( immunohistochemistry showed P<0.01; RT-PCR showed P<0.05); Compared to GM , the expression of Bax was declined in GR(P<0.01), but...