Studies Of The Protective And Mechanism Of Garlic On Alcohol-induced Subchronic Hepatotoxicity And The Effect On Learning And Memory Ability In Mouse

ObjectiveThe objective of this research was to demonstrate whether garlic could extenuate alcohol-induced male subchronic hepatotoxicity and neurotoxicity and by which mechanisms it worked. This study was also to provide the evidences for exploit garlic as healthy food and drug for the prevention and treatment of alcoholism.Methods1. The Protective Effect and Mechanism of Garlic on Alcohol-induced Subchronic Hepatotoxicity:60 ICR mice were randomly divided into six groups,each of ten. The mice in alcohol+high-dose Garlic, alcohol+middle-dose Garlic, alcohol+low-dose Garlic and alcohol+Vitamin E were pretreated intragastrically with 250 mg/kg, 500 mg/kg, 750 mg/kg garlic and 500 mg/kg Vitamin E, while others were given distilled water instead. One hour later, animals were administered 50% alcohol (15ml/kg) orally once a day; control animals were given distilled water only. After 28 days, the mice were killed and the samples of liver were taken for test.2. The Protective Effect and Mechanism of Garlic on the Effect of Learning and Memory ability induced by Alcohol:The grouping of animals and the scheme of intervention are same as the above experiment. The mice were taken the test of Morris Water Maze respectively 28 days before and after the intervention. At the same time, we measured the total scores and...