Experimental Study Of The Facial Nerve Paralysis Induced By Reactivation Of Latent Herpes Simplex Type-1 Infection In Mice

Objective:To establish an animal model of Bell's palsy induced by the reactivation of latent herpes simplex virus type 1 ,and discussion the relationship of the virus between with bell's palsy, to find the etiopathogenisis and mechanisms of bell's palsy clearly .Methods: 1 150 four-week-old female Balb/c mice weighted 16-18 gram were seleceted . to use scratching the surface of bilateral auricles by a 26-gauge needle, 25μl HSV-1 with a titer of 6.7×10~7 PFU/ml was inoculated into the right auricle, and the same volume of PBS was placed in the left. After this treatment observe the mice whether or not palsy everyday by the three aspects blink reflex ,vibrissae movement and apex of nose location;2 Eight weeks after recovery from facial nerve paralysis caused by inoculation with HSV-1, the mice(90) were divided into A, B and C groups, the mice in A treated with auricular skin scratch at the site of the previous inoculation combination with intraperitoneal injection of cyclophosphamide with dose of 80mg/kg/d in 3 days ,B with with only intraperitoneal injection of cyclophosphamide and C with intraperitoneal injection of Sodium Chloride with the same dose . After this treatment observe the mice whether or not palsy everyday, they were sacrificed when the mice were facial paralysis. If the mice were not facial paralysis,they were sacrificed until 20days The HSV-1 DNA in bilateral facial nerve, bilateral trigeminal ganglion after the treatment is over was...