The Effect Of Artificial Liver On Serum Cytokines In Pig Model Of Fulminant Hepatic Failure

Objective:Artificial liver is a technique which have gained great progress in both foundation and clinic for the past few years. Yet there is great difficultity in evaluating it objectively, because progress of fulminant hepatic failure is very quick;clinical conditions vary wonderfully; and there are a great deal of influential factors. Therefore we established animal model of fulminant hepatic failure to investigate the effect of plasma exchange (PE) combined with continuous hemofiltration (CHF) on the dynamic change of serum cytokine through the determination of the varient of cytokines in the treatment group and control group. And then to evaluate the effectiveness, possible mechanism and optimal treatment intervals of artificial liver.Methods:. Eleven minipigs were divided into two groups at random: control group (n=5) and treatment group (n=6) . And another five minipigs which weighs 18-26 kilogrames are selected to provide the nomal plasma needed for plasma exchange. All of the control group and treatment group animals were induced fulminant hepatic failure with administration of D-galactosamine (dosage, 1.2g/kg ) through the intubation remained in external jugular vein in 10 minutes. 24 hours and 48 hours later the treatment group was given PE combined with HF. The PE method is as follow: draw blood from the intubation remained in external jugular vein, and drain the plasma isolated from the plasma disjunctor, and at the same time fresh frozen plasma were put into the...