TGF-β₁ And NF-κB Expression Of Conjunctiva Endepidermis Cells In Diabetes Mellitus Patients Xerophthalmia

Objective: To investigate Diabetes mellitus (DM) patients lacrimal film function change system, by studing transforming growth factor-β₁ (TGF-β₁) and nuclear factor-κB (NF-κB) expression of DM patients conjunctiva endepider-mis cells.Methods: We selected randomly Type 2 DM patients and healthy adults with total 162 patients: DM patients 92(92 eyes), control group 70(70 eyes); and we selected randomly one eye to study. We undertook tear film break-up time(BUT), Schirmer I-test, Fluorescein staining. And we determined TGF-β₁, and NF-κB. expression of these patients bulbar conjunctiva endeidermis specimens by using blotting and immunohistochemistry way.Results: 1.The rate of BUT value with DM < 10s, and Schirmer value <5mm increased obviously(X²=15.19, P<0.01; X²=7.89, P<0.01). And DM cornea fluorescein staining positive expression was higher than control group (X²=7.86, P<0.05). 2. NF-κB and TGF-β₁ expression of DM staining level was stronger than control group(X²=17.58, P<0.01; X²=23.52, P<0.001). 3.NF-κB and TGF-β₁ expression of DM group correlated negatively with BUT value(r=-0.914,...