Effect Of Adrenaline On Nitric Oxide Synthase In Aorta Endothelial Cell Of Rats In Cold And Its Significance In Inducing Cold-adaptation

Objective: To clarify the mechanism of cold-adaptation establishment and explore effective medical measures to accelerate cold-adaptation formation and prevent and cure cold injury, the effect of increased adrenaline induced by cold exposure on activity of NOSin aortal endothelial cells of cold-injury and cold-adaptation rats was studied. Methods:The rat model of cold injury and adaptation was established. Endothelial cells of aorta were cultured and used for the establishment of cell stress model. NOS activity of aortal endothelium in cold exposure rats was detected, and L-Arg was used to regulate NOS activity of aortal endothelium in cold stressed rats. Mental training was used to induce human cold adaptation. In these models, the concentration of adrenaline in plasma was analyzed by enzyme-linked immunosorbent assay and NOS activity of aortal endothelial cells was measured by nitrate reductase assay, LDH activity in serum and aortal endothelial cell culture media was detected with ultraviolet spectrophotometer and the level of NOS mRNA in aortal endothelial cells of rats was evaluated by reverse transcription PCR.Results: The elevated adrenaline level and decreased activity of NOS in aortalendothelial cells were found in cold stress rats. The adrenaline concentration was significantly lower and the NOS activity was much higher after cold exposure in cold adaptation rats, meanwhile, the degree of cold injury decreased dramatically in cold adaptation rats. L-Arg could antagonize the decrease of NOS activity in ndothelial cells of aorta and alleviate injury . One of non-specific adaptation induced by restraint stress had the same effect as that of cold adaptation on adrenaline concentration and NOS activity. Mental training in human promoted cold adaptation by increasing the NOS activity.Conclusion: Adr-NOS is an important mechanism for the establishment of cold adaptation. Restraint stress accelerates cold adaptation by regulating NOS activity via...