Study On Reproductive And Developmental Abnormalities In Hypospadiac Male Rats Induced By Maternal Exposure To Di-n-butyl Phthalate During Late Pregnancy

Objective: The objective of this study was to establish a hypospadiac rat model by maternal exposure to di-n-butyl phthalate (DBP) during late pregnancy and evaluate the reproductive and developmental abnormalities of their hypospadiac male offspring.Methods: Timed-pregnant Sprague-Dawley rats were given DBP by gastric intubation at doses of 0(Group A), 500(Group B), 800(Group C) or 1200(Group D) mg/kg from gestation day (GD) 14 to GD18, with 10 dams a group. Each rat of the garage capacity was 1ml/day and maternal body weight was recorded daily. After all pregnant rats had delivered, the body weight and number of male offspring were counted in each group. On postnatal day (PND) 1 and PND 7 the anogenital distance (AGD) were measured with anatomy microscope. On PND 7 the incidence of hyposapdias was evaluated and Transverse serial histologic changes of hypospadiac genitalia was also checked. On PND 70 the number of cryptorchidism was counted in each group. After anatomy, the...