Study On The Apoptosis Of Gastric Cancer SGC-7901 Cells Induced By Dimethylarsinic Aci

Objective:To observe the effects of dimethylarsinic acid (DMAA) on gastric cancer SGC-7901 cells and investigates its mechanism primarily.Methods:After coculturing SGC-7901 cells with different concentra- tions of DMAA, the inhibitory ratio, morphological changes and biological changes were detected by MTT assay, Laser Scanning Confocal Micro- scopy and DNA gel electrophoresis, respectively.Results:The effective concentrations of DMAA inducing apoptosis are from 0.25mmol/L to 5.0mmol/L. Morphological changes including conden- sation of cellular chromatin and fragmentation of nuclear and apoptotic bodies were observed. Fragmentation of internucleosomal DNA was very apparent on electrophoretic patterns at this concentrations, both of which are typical features of apoptosis, while at high concentrations (≥10mmol/L) DMAA caused mainly necrosis. At the same time, MTT assay indicates that the cytoactivation decreases correspondingly with the concentrations increase.Conclusions:The results suggest that DMAA induces apoptosis of SGC- 7901 cells at low concentrations, while at high concentrations DMAA mainly cause gastric cancer cells necrosis.