Comparative Studies By Five Methods And Modificative Application On Detection Of Platelet Antibodies

Objective: Improvements in the availability and quality of platelet transfusions have markedly reduced the morbidity and mortality associated with intensive myelosuppressive therapy. Transfusion of platelet concentrates (PC) reduced the incidence of fatal hemorrhages in several thrombocytopenic conditions. Unfortunately, long-term platelet supportive care may be complicated by the development of a state of refractoriness, resulting in inadequate recovery of functional platelets. PC handling, clinical conditions of the patients and alloimmunization are the main factors affecting refractoriness. So, nowadays one of the main limiting factor in platelet transfusion therapy is the development of alloimmunization. Alloimmunization and refractoriness to platelet transfusion will remain a significant clinical problem in the long term. So it is very important for us to find a useful method to determine the specificity of patients' platelet antibodies and be of benefit of to patients of platelet transfusions.Methods: As most of antibodies are Hal's, to 67 unrelated cases, several serologic assays have been comparatively studied: A solid-phase, HLA-specific enzyme-linked immunosorbent assay (ELISA) that detects the presence of immunoglobulin (Ig) G anti-HLA antibodies uses purified HLA antigens prepared by affinity column chromatography from several hundred...