Topography Of Ganglion Cell In Retina Of Sprague-Dawley Rat

Objective The retina is a filmy piece of brain tissue with ability of visual perception, and retinal ganglion cells (RGCs) are it's terminal output neurons and play a relatively important role in the process in which retina information has been integrating and transporting. It's very important for the central visual system that the difference in distribution of photoreceptors (cone- and rod-cells), bipolar cells and RGCs with different latitudes in retina, especially the density of RGC is for the amount of information projecting to the brain. As to the researches of RGCs, there were much many reports whatever in or out of our country, but with much difference in them. Furthermore, there is never, up to today, a report on the topography of RGCs analyzed in the whole-mount retina of rats. In this research, RGCs were retrogradely labeled by injecting Fluoro-Gold (FG) into bilateral superior colliculus (CS) and dorsal lateral geniculate body nucleus (dLGN) of the Sprague-Dawley (S-D) rats to reveal the distribution of RGC and have the topography of them analyzed in the whole-mount retina in S-D rat, label the projecting sites from retina in brain, and thus go forward to reveal the different physiological functions to transport optic information, and afford references to researches of human visual science.Materials and Methods 10g/L FG in 0.1mol/L phosphate buffered saline ( PBS, pH 7.4 ) containing 10g/L dimethyl sulfoxide (DMSO) was prepared forthwith. Opened the skull and retrogradely labeled RGCs by injecting FG into bilateral CS and dLGN in adult S-D rats. Four weeks later, the rats were perfused through the heart with 0.1mol/L PBS (pH 7.4) and a fixative consisting of 4% paraformaldehyde (in...