Inhibition Of KDR Gene Expression In Human Prostate Carcinoma PC-3 Cells

Vascular endothelial growth factor receptor-2/ KDR(VEGFR2, also named as kinase-insert domain containing receptor: KDR) is diffusively expressed in endothelial cells,partially in some tumor cells.and appears to play a pivotal role on promoting the tumor vascularization,tumor growth and metastasis.VEGF plays its role throuth paracrine or autocrine mechanism.(1) The paracrine stimulation of angiogenesis:solid tumor cells secret VEGF, which binds to KDR on vascular endothelial cells and activates the signaling pathway,resulting in the endothelial cell proliferation,neovascularization and the increase of vascular permeability.(2) The autocrine stimulatory effect on tumor proliferation:VEGF is an autocrine growth factor for some KDR positive human tumors,such as prostate cancer.The exsting VEGF-KDR autocrine loops promote tumor cell proliferation directly. Inhibition of KDR expression have the potential to inhibit tumor growth on two levels:the level of the anigogenesis and the level of tumor cell.KDR is the suitable target molecule for gene therapy.RNA interference (RNAi) refers to post-transcriptional silencing induced by double-stranded RNA (dsRNA).After introduced cells,the exogenous dsRNA is cleaved into 19- to 23-nt RNA known as small interference RNA (siRNA) by Dicer.After the siRNA have been incorporated into a ribonuclease...