Mechanism Of N-acety-L-cysteine Therapy Acute Necrotizing Pancreatitis Of Experiment Rats

OBJECTIVE This study was to investigate the effects of N-acety-L-cysteine on transcription factor nuclear - κB (NF-κB),I -κB activity of pancreatic cells and inflammatory cytokines (IL-6, TNF-a and ICAM-1) of blood in acute necrotizing pancreatitis(ANP) of rats.METHODS Rats of ANP were induced by retrograde injection of 5% sodiumtaurocholate into the pancreatic duct.Sixty male-rats were randomly divided into three groups, namely group A , group B and group C. There were four time points for every group (namely at 1hr, 3hr, 5hr, and 7hr) and five rats at every time point. Group A: ANP(-) contrast group; Group B: ANP(+) contrast group; Group C: ANP(+) NAC treatment group. Rats of ANP were killed after observed at 1hr, 3hr, 5hr, 7hr and The AMS of blood, hydropericardium and urine were measured respectively. Lung tissue, pancreas tissue were futher pathologied. NF-κB activity in the cell nuclear of pancreatic acinar cells and I κ Ba activity in the cell spasm of pancreatic acinar cells were measured by immunohistocheffiistry. The concentrations of inflammatory cytokines (IL-6, TNF-a and ICAM-1) of the blood were measured by ELISA.RESULTS N-acety-L-cysteine can decrease the damage of preacreas and lung tissue. NAC can significantly inhibit the NF-κB activity and stablize I-κB activity.ELISA indicated that the concentrations of inflammatory cytokines (IL-6, TNF-a and ICAM-1) in the blood of ANP(+) rats significantly increased at 3hr and the top of it at 5hr and decreased at 7hr. The concentrations of inflammatory cytokines (IL-6, TNF-a and...